How does citrate from the citric acid cycle affect glycolysis?

There are specific enzymes for different reactions of different substrates or molecules. Inhibition of an enzyme can cause the reaction to cease.

• Glycolysis and citric acid cycle are the pathways involve for the catabolism of a carbohydrate. The two pathways are connected and a certain inhibition of an enzyme would stop or slow down the whole process.

A citrate in the citric acid cycle is formed from oxaloacetate and acetyl-CoA. The citrate can go from the mitochondria to the cytosol. Citrate in the cytosol is a known inhibitor of phosphofructokinase-1, the rate-limiting enzyme in glycolysis. The inhibition of phosphofructokinase-1 by citrate signals enough energy in the system. Therefore, it signals the pathway that it can slow down the oxidation of glucose molecules for energy purposes.