(a) In the control sample histogram, identify the phase of the cell cycle (G1, S, or G2) of the population of cells in each region delineated by vertical lines. Label the histogram with these phases and explain your answer. (b) Does the S phase population of cells show a distinct peak in the histogram? Why or why not?

Short Answer

Expert verified

(a) According to the diagram, A, B, and C are intervals in which cells are grown in culture, and G1, G2, and S phases occur in a sequential manner. So interval A shows the G1 phase, interval B shows the S phase, and interval C shows the G2 phase.

(b) As the S phase progresses, the DNA content in each cell changes. Thus, here the cells in the S phase are showing different levels of fluorescence.

Step by step solution

01

Step 1:Cell cycle

The cell cycle is a collection of sequential steps by which cells divide to produce two daughter cells. It completes in four phases that are G1, S, G2, and M. G1 is the first phase at which cells do all preparations for division.

In the S phase, the cell replicates its DNA. In the G2 phase, cells are prepared to divide. M phase is a dividing phase.

02

Explanation for answer (a)

Cells are inhibited from dividing in interval A when they start their cell cycle. The cell cycle initiates from G1 and then to S, and after it, they reach in G2 phase.So in interval A, G1 is the phase, and interval B indicates the S phase, and C shows the G2 phase.

03

Explanation for answer (b)

The cells in the S phase do not show a distinct peak in the histogram because the DNA content varies in each cell as it progresses to divide. Due to different levels of fluorescence in each cell, the peak is not formed distinct.

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Most popular questions from this chapter

Cell A has as much DNA as cells B, C, and D in mitotically active tissue. Cell A is most likely in

(A) G1.

(B) G2

(C) prophase.

(D) metaphase

One difference between cancer cells and normal cells is that cancer cells

(A) are unable to synthesize DNA.

(B) are arrested at the S phase of the cell cycle.

(C) continue to divide even when they are tightly packed together.

(D) cannot function properly because they are affected by density-dependent inhibition.

The histogram representing the treated sample shows the effect of growing the cancer cells alongside human umbilical cord stem cells that produce the potential inhibitor. (a) Label the histogram with the cell cycle phases. Which phase of the cell cycle has the greatest number of cells in the treated sample? Explain. (b) Compare the distribution of cells among G1, S, and G2 phases in the control and treated samples. What does this tell you about the cells in the treated sample? (c) Based on what you learned in Concept 12.3, propose a mechanism by which the stem cell-derived inhibitor might arrest the cancer cell cycle at this stage. (More than one answer is possible.)

The decline of MPF activity at the end of mitosis is due to

(A) the destruction of the protein kinase CDK.

(B) decreased synthesis of Cdk.

(C) degradation of cyclin

(D) accumulation of cyclin.

Although both ends of a microtubule can gain or lose subunits, one end (called the plus end) polymerizes and depolymerizes at a higher rate than the other end (the minus end). For spindle microtubules, the plus ends are in the center of the spindle, and the minus ends are at the poles. Motor proteins that move along microtubules specialize in walking either toward the plus end-directed and minus end-directed motor proteins, respectively. Given what you know about chromosome movement and spindle changes during anaphase, predict which type of motor proteins would be present on (a) kinetochore microtubules and (b) non-kinetochore microtubules.

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