Assume you are mapping genes A, B, C, and D in Drosophila. You know that these genes are linked on the same chromosome, and you determine the recombination frequencies between each pair of genes to be as follows: A-B, 8%; A-C, 28%; A-D, 25%; B-C, 20%; B-D, 33%.

1. Describe how you determined the recombination frequency for each pair of genes.

2. Draw a chromosome map based on your data.

The number of recombinants produced concerning the number of offspring in a cross is referred to as recombinant frequency.The recombination frequency between gene pairs helps to calculate the distance between the genes on a chromosome.

1% recombinant frequency refers to one map unit. The distance between the genes on the chromosome is referred to as map units.

Calculate the recombinant frequency for geneAand geneB. First, a homozygous dominant parent (AABB) is crossed with a recessive parent (aabb). The offspring produced would be F1dihybrid with genotypeAbBb.

The F1dihybrid is then test crossed with a homozygous recessive fly (aabb).The offspring produced from this cross is observed and segregated as parental and recombinants based on parents' genotype.

The recombination frequency between the gene pairs is calculated using the formula:

$RecombinantFrequency=\frac{Numberofrecombinants}{Totalnumberofoffspring}\times 100$

According to the given data, the distance between geneAand geneBis 8 map units, betweenAandCis 28 map units, betweenAandDis 25 map units, betweenBandCis 20 map units, and betweenAandDgene is 33 map units.

The data suggest that gene A and B is located between gene D and C. Therefore, the sequence of genes on the chromosome is DABC.