Chapter 11: Problem 11
During DNA replication, which enzyme can be disposed of in an organism with a mutant DNA polymerase that does not require a free \(3^{\prime}-\mathrm{OH} ?\)
Chapter 11: Problem 11
During DNA replication, which enzyme can be disposed of in an organism with a mutant DNA polymerase that does not require a free \(3^{\prime}-\mathrm{OH} ?\)
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Get started for freeSuppose that \(E .\) coli synthesizes DNA at a rate of 100,000 nucleotides per minute and takes 40 minutes to replicate its chromo- some. (a) How many base pairs are present in the entire \(E .\) coli chromosome? (b) What is the physical length of the chromosome in its helical configuration- that is, what is the circumference of the chromosome if it were opened into a circle?
During replication, what would be the consequences of the loss of functions of (a) single-stranded binding proteins, (b) DNA ligases, (c) DNA topoisomerases, and (d) DNA helicases?
Summarize and compare the properties of DNA polymerase I II, and III.
What would be the impact of the loss of processivity on DNA Pol III?
Describe the role of \(^{15} \mathrm{N}\) in the Meselson-Stahl experiment.
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