Chapter 13: Problem 16
Most proteins have more leucine than histidine residues, but more histidine than tryptophan residues. Correlate the number of codons for these three amino acids with this information.
Chapter 13: Problem 16
Most proteins have more leucine than histidine residues, but more histidine than tryptophan residues. Correlate the number of codons for these three amino acids with this information.
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Get started for freePresent an overview of various forms of posttranscriptional processing in eukaryotes. For each, provide an example.
In a mixed copolymer experiment, messages were created with either \(4 / 5 \mathrm{C}: 1 / 5 \mathrm{A}\) or \(4 / 5 \mathrm{A}: 1 / 5 \mathrm{C}\). These messages yielded proteins with the following amino acid compositions. Using these data, predict the most specific coding composition for each amino acid.
In this chapter, we focused on the genetic code and the transcription of genetic information stored in DNA into complementary RNA molecules. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions: (a) Why did geneticists believe, even before direct experimental evidence was obtained, that the genetic code would turn out to be composed of triplet sequences and be nonoverlapping? Experimentally, how were these suppositions shown to be correct? (b) What experimental evidence provided the initial insights into the compositions of codons encoding specific amino acids? (c) How were the specific sequences of triplet codes determined experimentally? (d) How were the experimentally derived triplet codon assignments verified in studies using bacteriophage MS2? (e) What evidence do we have that the expression of the information encoded in DNA involves an RNA intermediate? (f) How do we know that the initial transcript of a eukaryotic gene contains noncoding sequences that must be removed before accurate translation into proteins can occur?
Sydney Brenner argued that the code was nonoverlapping because he considered that coding restrictions would occur if it were overlapping. A second major argument against an overlapping code involved the effect of a single nucleotide change. In an overlapping code, how many adjacent amino acids would be affected by a point mutation? In a nonoverlapping code, how many amino acid(s) would be affected?
Substitution RNA editing is known to involve either C-to-U or A-to-I conversions. What common chemical event accounts for each?
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