How can you determine whether a particular gene is being transcribed in different cell types?

Question: Describe the process of determining whether a specific gene is being transcribed in different cell types. Answer: To determine whether a specific gene is being transcribed in different cell types, follow these steps: 1) Isolate total RNA from each cell type. 2) Convert the extracted RNA to complementary DNA (cDNA) through reverse transcription. 3) Employ polymerase chain reaction (PCR) or quantitative PCR (qPCR) to assess the presence of the target gene in the cDNA generated from different cell types. 4) Alternatively, use other methods such as RNA-Seq or DNA microarrays to assess the presence and abundance of specific gene transcripts. 5) Interpret the results to draw conclusions about the gene's transcription status and its role in specific cell types. Further analysis and experimental validation may be required to investigate the gene's functional importance.