Chapter 18: Problem 5
Distinguish between the syncytial blastoderm stage and the cellular blastoderm stage in Drosophila embryogenesis.
Chapter 18: Problem 5
Distinguish between the syncytial blastoderm stage and the cellular blastoderm stage in Drosophila embryogenesis.
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Get started for freeExperiments have shown that any nuclei placed in the polar cytoplasm at the posterior pole of the Drosophila egg will differentiate into germ cells. If polar cytoplasm is transplanted into the anterior end of the egg just after fertilization, what will happen to nuclei that migrate into this cytoplasm at the anterior pole?
Nuclei from almost any source may be injected into Xenopus oocytes. Studies have shown that these nuclei remain active in transcription and translation. How can such an experimental system be useful in developmental genetic studies?
In this chapter, we have focused on large-scale as well as the inter- and intracellular events that take place during embryogenesis and the formation of adult structures. In particular, we discussed how the adult body plan is laid down by a cascade of gene expression, and the role of cell-cell communication in development. Based on your knowledge of these topics, answer several fundamental questions: (a) How do we know how many genes control development in an organism like Drosophila? (b) What experimental evidence demonstrates that molecular gradients in the egg control development? (c) How did we discover that selector genes specify which adult structures will be formed by body segments? (d) How did we learn about the levels of gene regulation involved in vulval development in \(C .\) elegans? (e) How do we know that eye formation in all animals is controlled by a binary switch gene?
Much of what we know about gene interactions in development has been learned using nematodes, yeast, flies, and bacteria. This is due, in part, to the relative ease of genetic manipulation of these well-characterized genomes. However, of great interest are gene interactions involving complex diseases in humans. Wang and White (2011. Nature Methods 8(4) \(341-346\) ) describe work using RNAi to examine the interactive proteome in mammalian cells. They mention that knockdown inefficiencies and off-target effects of introduced RNAi species are areas that need particular improvement if the methodology is to be fruitful. (a) How might one use RNAi to study developmental pathways? (b) Comment on how "knockdown inefficiencies" and "off-tar-get effects" would influence the interpretation of results.
How can you determine whether a particular gene is being transcribed in different cell types?
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