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Chapter 16: Problem 12

Describe the role of attenuation in the regulation of tryptophan biosynthesis.

Short Answer

Expert verified
Attenuation plays a crucial role in the regulation of tryptophan biosynthesis by controlling the transcription of the trp operon, a group of genes involved in tryptophan biosynthesis. When tryptophan levels are high, attenuation stops the transcription of the trp operon, preventing the production of enzymes needed for tryptophan biosynthesis. This regulation helps maintain an optimal balance of tryptophan in bacteria and ensures the conservation of resources.

Step by step solution

01

Define Tryptophan Biosynthesis

Tryptophan is an essential amino acid in living organisms, which means that our bodies cannot produce it and we must obtain it from our diet. In bacteria, tryptophan can be synthesized through a series of enzymatic reactions. The term "tryptophan biosynthesis" refers to the process in which bacteria produce tryptophan.
02

Regulation of Tryptophan Biosynthesis

Bacteria need to regulate the production of tryptophan to maintain an optimal balance. When tryptophan levels are high, the bacteria should stop the production of tryptophan to conserve resources. On the other hand, when tryptophan levels are low, the bacteria should ramp up tryptophan production to meet their needs. This regulation is tightly controlled at the level of gene expression, and one mechanism responsible for this control is called "attenuation."
03

Attenuation in Tryptophan Biosynthesis

Attenuation specifically refers to the process by which the synthesis of the tryptophan biosynthesis enzymes is controlled by regulating the transcription of the trp operon, which is a group of genes involved in tryptophan biosynthesis. When tryptophan levels are low, RNA polymerase (the enzyme responsible for transcribing DNA to mRNA) transcribes all the genes from the trp operon, creating the enzymes needed for tryptophan biosynthesis. However, when tryptophan levels are high, a process called "transcription attenuation" occurs, and the transcription of the trp operon is stopped prematurely, preventing the production of the enzymes for tryptophan biosynthesis. This transcription attenuation occurs due to the formation of a specific hairpin loop structure (also known as a stem-loop) in the mRNA during the transcription process. The presence or absence of tryptophan determines which hairpin loop forms – one loop promotes transcription termination, while the other allows transcription to continue. In summary, attenuation in tryptophan biosynthesis is a key regulatory mechanism that allows bacteria to respond to changing levels of tryptophan by modulating the transcription of the trp operon. This prevents wasteful enzyme production when tryptophan is abundant, and ensures a quick and effective response when tryptophan levels are low.

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Key Concepts

These are the key concepts you need to understand to accurately answer the question.

trp operon
The trp operon is a cluster of genes found in some bacteria responsible for the biosynthesis of the amino acid tryptophan. This operon is a major focus when exploring how cells control their internal production of essential molecules.

It works like this: when bacteria sense that tryptophan levels are low, the trp operon is activated. This leads to the production of enzymes that are needed to produce tryptophan from simpler starting materials. On the other hand, if cells have enough tryptophan, the operon is inactivated to conserve resources and energy. This decision of whether to turn on or off is a sophisticated process that involves a mechanism known as attenuation, which we're exploring in this context.
transcription regulation
Transcription regulation is akin to controlling whether a particular section of the DNA's instructions will be used to create proteins and enzymes.

Just like a complex assembly line, every step in manufacturing proteins from DNA instructions must be meticulously managed. In bacteria, the process begins when an enzyme called RNA polymerase latches onto the DNA. It travels along, reading the genetic blueprint and synthesizing messenger RNA (mRNA), a single-stranded molecule that carries the DNA's message to the places in the cell where proteins are made. But not all genetic messages should be read all the time; cells must respond to their environment. This is where attenuation, as seen in tryptophan biosynthesis, comes into play. Here, bacterial cells stop the mRNA manufacturing process early if tryptophan is plentiful, an ingenious example of transcription regulation.
amino acid synthesis
Amino acids are the building blocks for proteins and are critical for life. There are twenty standard amino acids, and while some can be synthesized by the human body, others, called essential amino acids, must be obtained from food. Bacteria, however, have the capability to manufacture all the amino acids they need, including tryptophan, through a series of reactions known as amino acid synthesis.

These reactions are catalyzed by enzymes that, in bacteria, are often produced in response to the availability of the end product. In the case of tryptophan, when its internal levels decline, bacteria synthesize more of the amino acid via enzymes produced by the trp operon. Understanding this process is not just about filling a cellular pantry; it's about grasping a life-sustaining dance of producing just enough, just in time.
gene expression control
Gene expression control is the grand orchestrator of cellular function, ensuring that genes are turned on or off at the correct times and in response to the needed cues. A cell's ability to adapt to its environment, grow, and reproduce hinges on this precise control.

This regulation can happen at any step in the gene expression process, from the initial unwinding of DNA to the final tweaks on a newly-made protein. However, one particularly elegant form of regulation happens at the stage of transcription, which we have seen exemplified in the attenuation mechanism within tryptophan biosynthesis. Through various signals, the cell can fine-tune its enzyme production, avoiding excess and scarcity, much like a conductor ensures the harmonious performance of an orchestra.

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Most popular questions from this chapter

What properties demonstrate that the lac repressor is a protein? Describe the evidence that it indeed serves as a repressor within the operon system.

Describe the experimental rationale that allowed the lac repressor to be isolated.

Neelaredoxin is a 15 -kDa protein that is a gene product common in anaerobic bacteria. It has superoxide-scavenging activity, and it is constitutively expressed. In addition, its expression is not further induced during its exposure to \(\mathrm{O}_{2}\) or \(\mathrm{H}_{2} \mathrm{O}_{2}\) [Silva, G. et al. \((2001) . J .\) Bacteriol. \(183: 4413-4420\) ]. What do the terms constitutively expressed and induced mean in terms of neelaredoxin synthesis?

Attenuation of the trp operon was viewed as a relatively inefficient way to achieve genetic regulation when it was first discovered in the 1970 s. since then, however, attenuation has been found to be a relatively common regulatory strategy. Assuming that attenuation is a relatively inefficient way to achieve genetic regulation, what might explain its widespread occurrence?

Both attenuation of the \(t r p\) operon in \(E\). coli and riboswitches in B. subtilis rely on changes in the secondary structure of the leader regions of mRNA to regulate gene expression. Compare and contrast the specific mechanisms in these two types of regulation with that involving short noncoding RNAs (sRNAs).
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