When disrupting a mouse gene by knockout, why is it desirable to breed mice until offspring homozygous \((-/-)\) for the knockout target gene are obtained?

Gene knockout is a technique used in research to study the functions of a specific gene, by deleting or inactivating the gene of interest in a living organism. For mice, this is often done through breeding, where two mice heterozygous \((+/-)\) for the knockout target gene are crossed to produce offspring. One goal of this breeding is to obtain homozygous \((−/−)\) offspring, which have both copies of the knockout target gene disrupted.

Obtaining homozygous \((−/−)\) offspring is crucial for several reasons: 1. Complete gene inactivation: Homozygous \((−/−)\) mice have both copies of the target gene inactivated or deleted, ensuring that the gene is completely non-functional. This allows researchers to clearly pinpoint the effects and functions of the knockout gene, without any interference from an active copy of the gene. 2. Phenotype observation: If the knockout target gene affects a specific phenotype in the mouse, homozygous \((−/−)\) offspring will allow researchers to observe the complete effect of the missing gene on that phenotype. 3. Reducing variability: By obtaining homozygous \((−/−)\) offspring, researchers can minimize the variability in their experiment results, as all of the mice being studied have the same genetic background and gene inactivation. 4. Control group comparison: Homozygous \((−/−)\) knockout mice can be used in conjunction with their wild-type \((+/+)\) counterparts as valid control groups. This allows researchers to make accurate comparisons and draw conclusions about the effects of the knockout gene.

To obtain homozygous \((−/−)\) offspring for the knockout target gene, researchers will typically cross two heterozygous \((+/-)\) mice. These heterozygous mice have one active copy and one disrupted copy of the target gene. According to Mendelian inheritance, the offspring will have the following distribution: - 25% homozygous wild-type \((+/+)\) - 50% heterozygous \((+/-)\) - 25% homozygous knockout \((−/−)\) Continued breeding of heterozygous mice will increase the chances of generating homozygous knockout \((−/−)\) offspring, allowing researchers to study the full effects of the disrupted gene.