Gene targeting and genome editing are both techniques for removing or modifying a particular gene, each of which can produce the same ultimate goal. Describe some of the differences between the experimental methods used for these two techniques.

The key differences between gene targeting and genome editing include the following: 1. Experimental methods: Gene targeting relies on homologous recombination using cloned DNA fragments with the desired modification, while genome editing techniques like CRISPR-Cas9 use a nuclease and a guide RNA to create targeted breaks. 2. Specificity and efficiency: Genome editing methods, such as CRISPR-Cas9, tend to have higher specificity and efficiency than gene targeting due to the introduction of double-strand breaks. 3. Applications and limitations: Gene targeting is primarily used for modifying single-copy genes in eukaryotic organisms, while genome editing has broader applications, including multiplexed gene editing and editing in prokaryotic organisms. Additionally, gene targeting requires specific recipient cell lines, whereas genome editing can be adapted to different cell types and organisms.