Chapter 17: Problem 9
What are the advantages of using a restriction enzyme whose recognition site is relatively rare? When would you use such enzymes?
Chapter 17: Problem 9
What are the advantages of using a restriction enzyme whose recognition site is relatively rare? When would you use such enzymes?
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Get started for freeList the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs and YACs provide over plasmids as cloning vectors?
The introduction of genes into plants is a common practice that has generated not only a host of genetically modified foodstuffs, but also significant worldwide controversy. Interestingly, a tumor-inducing plasmid is often used to produce genetically modified plants. Is the use of a tumor-inducing plasmid the source of such controversy?
List the steps involved in screening a genomic library. What must be known before starting such a procedure? What are the potential problems with such a procedure, and how can they be overcome or minimized?
To create a cDNA library, cDNA can be inserted into vectors and cloned. In the analysis of cDNA clones, it is often difficult to find clones that are full length-that is, many clones are shorter than the mature mRNA molecules from which they are derived. Why is this so?
List the steps involved in screening a genomic library. What must be known before starting such a procedure? What are the potential problems with such a procedure, and how can they be overcome or minimized?
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