Chapter 9: Problem 11
What are the structural differences between (a) purines and pyrimidines, and (b) ribose and deoxyribose sugars?
Chapter 9: Problem 11
What are the structural differences between (a) purines and pyrimidines, and (b) ribose and deoxyribose sugars?
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Get started for freeContrast the various contributions made to our understanding of transformation by Griffith, Alloway, and Avery.
What is the chemical basis of molecular hybridization?
What is the hyperchromic effect? How is it measured? What does \(T_{m}\) imply?
What component of the nucleotide is responsible for the absorption of ultraviolet light? How is this technique important in the analysis of nucleic acids?
Assume that you are interested in separating short \((25-40\) nucleotides) DNA molecules from a pool of longer molecules in the \(900-1000\) nucleotide range. You have two recipes for making your polyacrylamide gels; one recipe uses 12 percent acrylamide and would be considered a "hard gel," while the other uses 4 percent acrylamide and would be considered a loose gel. Which recipe would you consider using and why?
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