Chapter 12: Q2. (page 398)
The hypothetical elementary reaction has a rate constant of . What is the reaction velocity when the concentration of is ?
Short Answer
The reaction velocity for the hypothetical elementary reaction is 10-10Ms-1.
Chapter 12: Q2. (page 398)
The hypothetical elementary reaction has a rate constant of . What is the reaction velocity when the concentration of is ?
The reaction velocity for the hypothetical elementary reaction is 10-10Ms-1.
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Get started for freeYou are trying to determine the KM for an enzyme. Due to a labmishap, you have only two usable data points:
Substrate concentration (μM) | Reaction velocity (μM s⁻¹) |
1 | 5 |
100 | 50 |
Use these data to calculate an approximate value for KM. Is this valuelikely to be an overestimate or an underestimate of the true value? Explain.
Based on some preliminary measurements, you suspect that a sample of enzyme contains an irreversible enzyme inhibitor. You decide to dilute the sample 100-fold and remeasure the enzyme's activity. What would your results show if an irreversible inhibitor is present?
Explain why each of the following data sets from a Lineweaver Burk plot are not individually ideal for determining KM for an enzyme catalysed reaction that follows Michaelis-Menten kinetics.
Set A | 1/[S] (mM⁻¹) | 1/v0 (𝛍M⁻¹.s) |
0.5 | 2.4 | |
1.0 | 2.6 | |
1.5 | 2.9 | |
2.0 | 3.1 |
Set B | 1/[S] (mM⁻¹) | 1/v0(𝛍M⁻¹.s) |
8 | 5.9 | |
10 | 6.8 | |
12 | 7.8 | |
14 | 8.7 |
What distinguishes an inhibitor from an inactivator?
Why can’t enzyme kinetics prove that a particular enzyme mechanism is correct?
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