Log out Go to App
Go to App

Learning Materials

Features

Discover

Chapter 24: Q11P (page 876)

How is the melting curve of duplex DNA affected by decreasing the ionic strength of the solution?

Short Answer

Expert verified

The Tm value of the melting curve of duplex DNA decreases as the ionic strength of the solution decreases.

Step by step solution

01

The melting curve of DNA.

The denaturation features of DNA are shown by the melting curve of DNA, which is based on nabsorption at a particular wavelength. Melting temperature,Tm, is the specific heatat which halves of the DNA are denatured.

02

The effect of the melting curve of duplex DNA.

The electrons of phosphate groups are much more exposed to one other at lower ionic strength. As a result, they reject each other even more. Phosphate group repulsion destabilizes the double helix, lowering the melting curve of duplex DNA.

Hence, the Tm value of the melting curve of duplex DNA decreases as the ionic strength of the solution decreases.

Unlock Step-by-Step Solutions & Ace Your Exams!

  • Full Textbook Solutions

    Get detailed explanations and key concepts

  • Unlimited Al creation

    Al flashcards, explanations, exams and more...

  • Ads-free access

    To over 500 millions flashcards

  • Money-back guarantee

    We refund you if you fail your exam.

Start your free trial

Over 30 million students worldwide already upgrade their learning with Vaia!

One App. One Place for Learning.

All the tools & learning materials you need for study success - in one app.

Get started for free

Most popular questions from this chapter

In what nucleic acid conformation(s) does the ribose pucker with C2’ endo? C3’ endo?

Why does it make metabolic sense for UTP to inhibit carbamoylphosphate synthetase II, whereas ATP activates the enzyme?

Explain why most nucleotides adopt the anti conformation.

Infection with the influenza virus interferes with the normal expression of genes in the host cell. The viral protein M1 binds to histones electrostatically. (a) Would increasing the salt concentration promote or weaken M1–histone binding? (b) Would decreasing the pH promote or weaken M1–histone binding?

Describe the levels of DNA packaging in eukaryotic cells, from B-DNA to a metaphase chromosome
See all solutions

Recommended explanations on Biology Textbooks

Cellular Energetics

Read Explanation

Ecosystems

Read Explanation

Ecology

Read Explanation

Chemistry of Life

Read Explanation

Energy Transfers

Read Explanation

Biological Processes

Read Explanation
View all explanations

What do you think about this solution?

We value your feedback to improve our textbook solutions.

Study anywhere. Anytime. Across all devices.

Sign-up for free