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Chapter 24: Q26P (page 876)

Calculate the length of the 50,000 - bpDNA in a 30 - nmfiber.

Short Answer

Expert verified

The DNA in 30 - nm fibre has a length of 405 - nm .

Step by step solution

01

Calculation of contour length.

DNA coils around nucleosome residues during chromatin synthesis. By folding in a zigzag pattern, chromatin condenses even more to a 30 - nm diameter helical fibre.

The contour length must be calculated as follows:

50,000-bp×3.4A=170,000A

02

Determine the length of the DNA.

The initial coiling of the DNA around the histone cuts the length in half, and the creation of the 30 - nm fibre cuts the length in half again. As a result, the packing ratio of the 30 - nm fibre is as follows:7×6=42.

The DNA in 30 - nm fibre has the following length:

170,00042=4047Awhich is equal to 405 - nm.

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Most popular questions from this chapter

The enzyme ornithine decarboxylase generates a product that has been proposed to play a role in stabilizing compacted DNA. Draw the structure of the ornithine decarboxylase reaction product and explain how it interacts with DNA.

How many histone variants occur in human cells? How do they differ from each other? How does the histone variant CENP-A relate to chromosomal structure? Which histone variant is associated with X-chromosome inactivation?

Describe the levels of DNA packaging in eukaryotic cells, from B-DNA to a metaphase chromosome

E. coli ribosomes contain three RNA molecules named for their sedimentation behavior: 5S, 16S, and 23S. Draw a diagram showing the approximate positions of the three RNA species following electrophoresis.

Polyoma virus DNA can be separated by sedimentation in an ultracentrifuge (Section 5-2E) at neutral pH into three components that have sedimentation coefficients of 20, 16, and 14.5S and that are known as Types I, II, and III DNAs, respectively. These DNAs all have identical base sequences and molecular masses. In 0.15 M NaCl, both Types II and III DNA have melting curves of normal cooperativity and a Tm of 88°C. Type I DNA, however, exhibits a very broad melting curve and a Tm of 107°C. At pH 13, Types I and III DNAs have sedimentation coefficients of 53 and 16S, respectively, and Type II separates into two components with sedimentation coefficients of 16 and 18S. How do Types I, II, and III DNAs differ from one another? Explain their different physical properties.
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