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Chapter 3: Q14. (page 78)
By how many nucleotides, on average, do the genomes of two Homo sapiens differ?
There is a one-nucleotide difference in the genomes of two Homo sapiens.
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The adenine derivative hypoxanthine can base-pair with adenine. Draw the structure of this base pair.
Describe the outcome of a chain-terminator sequencing procedure in which (a) too few primers are present or (b) an excess of primers is present.
Why do cDNA libraries derived from different cell types within the same organism differ from each other?
A blood stain from a crime scene and blood samples from four suspects were analyzed by PCR using fluorescent primers associated with three STR loci: D3S1358, vWA, and FGA. The resulting electrophoretograms are shown below. The numbers beneath each peak identify the allele (upper box) and the height of the peak in relative fluorescence units (lower box).
(a) Since everyone has two copies of each chromosome and therefore two alleles of each gene, what accounts for the appearance of only one allele at some loci?
(b) Which suspect is a possible source of the blood?
(c) Could the suspect be identified using just one of the three STR loci?
(d) What can you conclude about the amount of DNA obtained from Suspect 1 compared to Suspect 4?
Question: What are some of the challenges of expressing a eukaryotic gene in a prokaryotic host cell?
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