Question: What are the advantages of PCR over traditional cloning?

Traditional cloning uses restriction endonucleases to create DNA fragments with complementary end sequences that may be linked together with a DNA ligase before being transformed.

Conventional cloning involves:

• Digesting double-stranded DNA inserts with restriction enzymes to form suitable ends.
• Purifying and isolating sufficient quantities.
• Ligating into a similarly treated vector of choice.

The polymerase chain reaction (PCR) is a commonly used method for rapidly making millions to billions of copies of a given DNA sample. It allows researchers to take a tiny sample of DNA and amplify it to a size big enough to investigate. PCR cloning has certain benefits over traditional cloning.

PCR allows for the production of billions of copies of a fragment of DNA in a matter of hours. PCR influences cloning technology by creating vast amounts of DNA that can be cloned.

PCR cloning is a fast way to clone genes, and it's frequently employed for tasks that require more coverage than standard cloning procedures can provide. It makes it possible to clone DNA fragments that aren't readily available in big quantities.