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Chapter 7: Q18. (page 219)
Many fish produce a tetrameric IgM. If each H chain has a mass of 70 kD, each light chain has a mass of 25 kD, and each J chain has a mass of 15 kD, what is the approximate mass of the IgM molecule?
The approximate mass of the IgM molecule is 775 kD.
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Which set of binding data is likely to represent cooperative ligand binding to an oligomeric protein?
(a)
| [Ligand] (mM) | Y |
| 0.1 | 0.3 |
| 0.2 | 0.5 |
| 0.4 | 0.7 |
| 0.7 | 0.9 |
(b)
| [Ligand] (mM) | Y |
| 0.2 | 0.1 |
| 0.3 | 0.3 |
| 0.4 | 0.6 |
| 0.6 | 0.8 |
An anemic individual, whose blood has only half the normal Hb content, may appear to be in good health. Yet a normal individual is incapacitated by exposure to sufficient carbon monoxide to occupy half of his/her heme sites (pCO of 1 torr for∼1 h; CO binds to Hb with 200-fold greater affinity than does O2). Explain.
Is myosin a fibrous protein or a globular protein? Explain.
Hemoglobin S homozygotes who are severely anemic often have elevated levels of BPG in their erythrocytes. Is this a beneficial effect?
Calculate the p50 value for hemoglobin if Yo2 = 0.82 when pO2 = 25 torr.
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