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Chapter 5: Q14P (page 127)

Identify the first residue obtained by Edman degradation of cytochrome c from (a) Drosophila, (b) baker’s yeast, and (c) wheat germ (see Table 5-6).

Short Answer

Expert verified

a) The first residue obtained by Edman degradation of cytochrome from Drosophila is glycine.

b) The first residue obtained by Edman degradation of cytochrome from baker’s yeast is threonine.

c) The first residue obtained by Edman degradation of cytochrome from wheat germ is none.

Step by step solution

01

Definition of Edman degradation

The Edman degradation is a chemical reaction that eliminates N-terminal amino acids from a peptide in a sequential way in a sequence of steps.

02

Step 2: Identify the first residue from Drosophila

Glycine is the first residue produced via Edman degradation of Drosophila cytochrome .

03

Step 3: Identify the first residue baker’s yeast

The first residue recovered by Edman's breakdown of baker's yeast cytochrome is threonine.

04

Step 4: Identify the first residue wheat germ

The first residue recovered from the Edman degradation of wheat germ cytochrome is none.

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Most popular questions from this chapter

What fractionation procedure could be used to purify protein 1 from a mixture of three proteins whose amino acid compositions are as follows?

1. 25% Ala, 20% Gly, 20% Ser, 10% Ile, 10% Val, 5% Asn, 5% Gln, 5% Pro

2. 30% Gln, 25% Glu, 20% Lys, 15% Ser, 10% Cys

3. 25% Asn, 20% Gly, 20% Asp, 20% Ser, 10% Lys, 5% Tyr

All three proteins are similar in size and pI, and there is no antibody available for protein 1.

You must cleave the following peptide into smaller fragments. Which of the proteases listed in Table 5-4 would be likely to yield the most fragments? The fewest?

NMTQGRCKPVNTFVHEPLVDVQNVCFKE

Electrospray ionization mass spectrometry (ESI-MS) of proteins involves creating positively charged ions of the protein and separating them according to their mass-to-charge ratio (m/z).

(a) What causes the different positive charges on different particles of the protein?

(b) The amino acid composition (in numbers of residues per chain) of hen egg-white lysozyme (HEWL) is as follows:

P 2 Y 3 N 14 H 1

D 7 M 2 L 8 E 2

C 8 R 11 G 12 F 3

A 12 I 6 K 6 V 6

S 10 W 6 T 7 Q 3

What is the maximum positive charge that can be present on a HEWL ion?

You must cleave the following peptide into smaller fragments. Which of the proteases listed in Table 5-4 would be likely to yield the most fragments? The fewest?

NMTQGRCKPVNTFVHEPLVDVQNVCFKE

You wish to sequence the light chain of a protease inhibitor from the Brassica nigra plant. Cleavage of the light chain by trypsin and chymotrypsin yields the following fragments. What is the sequence of the light chain?

Chymotrypsin

1. Leu–His–Lys–Gln–Ala–Asn–Gln–Ser–Gly–Gly–Gly–Pro–Ser

2. Gln–Gln–Ala–Gln–His–Leu–Arg–Ala–Cys–Gln–Gln–Trp

3. Arg–Ile–Pro–Lys–Cys–Arg–Lys–Phe

Trypsin

4. Arg

5. Ala–Cys–Gln–Gln–Trp–Leu–His–Lys

6. Cys–Arg

7. Gln–Ala–Asn–Gln–Ser–Gly–Gly–Gly–Pro–Ser

8. Phe–Gln–Gln–Ala–Gln–His–Leu–Arg

9. Ile–Pro–Lys

10. Lys
See all solutions

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