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Chapter 5: Q20P (page 128)

Draw a different phylogenetic tree based on the data in Problem 19.

Short Answer

Expert verified

The required phylogenetic tree is:

Problem presents data in a variety of ways.

Step by step solution

01

Explanation of Concept

A phylogenetic tree, also known as a phylogeny, is a diagram that shows how various species, animals, or genes descended from a common ancestor.

02

Drawing Phylogenetic trees

We compare amino acid sequences of given proteins to create phylogenetic trees. Proteins with more differences have evolved at a faster rate. Proteins and have only one difference, putting them near together on the phylogenetic tree, however Protein has three differences from both, putting it in a different branch.

Therefore, Problem presents data in a variety of ways.

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Most popular questions from this chapter

You wish to determine the sequence of a short peptide. Cleavage with trypsin yields three smaller peptides with the sequences Leu–Glu, Gly–Tyr– Asn–Arg, and Gln–Ala–Phe–Val–Lys. Cleavage with chymotrypsin yields three peptides with the sequences Gln–Ala–Phe, Asn–Arg–Leu–Glu, and Val–Lys–Gly–Tyr. What is the sequence of the intact peptide?

Identify the first residue obtained by Edman degradation of cytochrome c from (a) Drosophila, (b) baker’s yeast, and (c) wheat germ (see Table 5-6).

You wish to sequence the light chain of a protease inhibitor from the Brassica nigra plant. Cleavage of the light chain by trypsin and chymotrypsin yields the following fragments. What is the sequence of the light chain?

Chymotrypsin

1. Leu–His–Lys–Gln–Ala–Asn–Gln–Ser–Gly–Gly–Gly–Pro–Ser

2. Gln–Gln–Ala–Gln–His–Leu–Arg–Ala–Cys–Gln–Gln–Trp

3. Arg–Ile–Pro–Lys–Cys–Arg–Lys–Phe

Trypsin

4. Arg

5. Ala–Cys–Gln–Gln–Trp–Leu–His–Lys

6. Cys–Arg

7. Gln–Ala–Asn–Gln–Ser–Gly–Gly–Gly–Pro–Ser

8. Phe–Gln–Gln–Ala–Gln–His–Leu–Arg

9. Ile–Pro–Lys

10. Lys

What fractionation procedure could be used to purify protein 1 from a mixture of three proteins whose amino acid compositions are as follows?

1. 25% Ala, 20% Gly, 20% Ser, 10% Ile, 10% Val, 5% Asn, 5% Gln, 5% Pro

2. 30% Gln, 25% Glu, 20% Lys, 15% Ser, 10% Cys

3. 25% Asn, 20% Gly, 20% Asp, 20% Ser, 10% Lys, 5% Tyr

All three proteins are similar in size and pI, and there is no antibody available for protein 1.

Describe the basis for separating proteins by ion exchange, hydrophobic interaction, gel filtration, and affinity chromatography.
See all solutions

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