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Chapter 21: Problem 19

\(\mathrm{PCR}\) a. uses RNA polymerase. b. takes place in huge bioreactors. c. uses DNA replication in vitro. d. makes many nonidentical copies of DNA. e. All of these are correct.

Short Answer

Expert verified
The correct statement is: \(c. PCR \) uses DNA replication in vitro.

Step by step solution

01

Analyze statement (a)

PCR uses RNA polymerase. This statement is incorrect. PCR uses DNA polymerase, not RNA polymerase, to replicate DNA.
02

Analyze statement (b)

PCR takes place in huge bioreactors. This statement is also incorrect. PCR takes place in a small instrument called a thermocycler, which precisely changes temperature during the PCR process.
03

Analyze statement (c)

PCR uses DNA replication in vitro. This statement is correct. PCR is an in vitro (test tube) method that is used to amplify specific DNA sequences.
04

Analyze statement (d)

PCR makes many nonidentical copies of DNA. This statement is incorrect. PCR makes many identical copies of a specific DNA sequence, not nonidentical copies.
05

Analyze statement (e)

All of these are correct. Since not all of the above statements are correct, this statement is incorrect.
06

Conclusion

Based on our analysis, the correct statement is: c. PCR uses DNA replication in vitro.

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Most popular questions from this chapter

The enzyme responsible for adding new nucleotides to a growing DNA chain during DNA replication is a. helicase. b. RNA polymerase. c. DNA polymerase. d. ribozymes.

The process of converting the information contained in the nucleotide sequence of RNA into a sequence of amino acids is called a. transcription. b. translation. c. translocation. d. replication.

What is the polymerase chain reaction (PCR), and how is it used to produce multiple copies of a DNA segment? (page 505 )

The double-helix model of DNA resembles a twisted ladder in which the rungs of the ladder are a. complementary base pairs. b. A paired with G and C paired with T. c. A paired with \(\mathrm{T}\) and \(\mathrm{G}\) paired with \(\mathrm{C}\) d. a sugar-phosphate paired with a sugar-phosphate. e. Both a and c are correct.

RNA processing a. removes the introns, leaving only the exons. b. is the same as transcription. c. is an event that occurs after RNA is transcribed. d. is the rejection of old, worn-out RNA. e. Both a and c are correct.
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