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Chapter 4: Problem 3

Transcribe and translate the following DNA sequence (nontemplate strand): 5'-ATGGCCGGTTATTAAGCA-3'

Short Answer

Expert verified
Answer: The amino acid sequence of the protein translated from the given nontemplate DNA strand is Methionine - Alanine - Glycine - Tyrosine (Met-Ala-Gly-Tyr or M-A-G-Y).

Step by step solution

01

Find the template strand

The nontemplate strand is given as 5'-ATGGCCGGTTATTAAGCA-3'. To find the template strand, write the complementary DNA strand, switching A with T and G with C (and vice versa): 5'-TACCGGCAATATTGCT-3' (nontemplate strand) 3'-ATGGCCGGTTATTAAGCA-5'(template strand)
02

Transcribe the template strand into mRNA

Replace the T with U (as mRNA contains uracil instead of thymine), to generate the mRNA sequence based on the template strand: mRNA: 5'-AUGGCCGGUUAUUAAGCA-3'
03

Translate the mRNA into an amino acid sequence

Divide the mRNA sequence into codons (3-nucleotide groups) and find the corresponding amino acid for each codon using the genetic code: AUG: Methionine (Met or M) GCC: Alanine (Ala or A) GGU: Glycine (Gly or G) UAU: Tyrosine (Tyr or Y) UAA: Stop Codon (*) Therefore, the amino acid sequence is: Methionine - Alanine - Glycine - Tyrosine (Met-Ala-Gly-Tyr or M-A-G-Y). The protein translation stops at the stop codon.

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Most popular questions from this chapter

Describe the structure and complementary base pairing of DNA.

Describe the organization of the eukaryotic chromosome.

The RNA components of ribosomes are synthesized in the ________. a. cytoplasm b. nucleus c. nucleolus d. endoplasmic reticulum

Prokaryotes contain a ________chromosome, and eukaryotes contain ________ chromosomes. a. single-stranded circular; single-stranded linear b. single-stranded linear; single-stranded circular c. double-stranded circular; double-stranded linear d. double-stranded linear; double-stranded circular

Which of the following does cytosine pair with? a. guanine b. thymine c. adenine d. a pyrimidine
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