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Chapter 17: Q. 17.71 (page 607)

How are plasmids obtained from $E .$ coli?

Short Answer

Expert verified

Electroporation process is used to obtain plasmids from $E .$ coli.

Step by step solution

Given Information

We need to find out how plasmids obtained from $E .$ coli.

Explanation

Electroporation is used to transfer a plasmid into competent Escherichia coli (E.coli) cells, which are then plated out on solid media and allowed to divide and grow. The concept and necessity of gene transformation in genetic engineering can be grasped through experiment.

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Most popular questions from this chapter

Endorphins are polypeptides that reduce pain. What is the amino acid order for the endorphin leucine enkephalin(leu-enkephalin), which has the following mRNA? $(17.5)$

AUG UAC GGU GGA UUU CUA UAA

Write the complementary base sequence for each of the following DNA segments:

a. $G A C T T A G G C$
b. $T G C A A A C T A G C T$
c. $A T C G A T C G A T C G$

What bases in mRNA are used to complement the bases $A$ , $T$ , $G$ , and $C$ in DNA?

In the genetic disease uridine monophosphate synthase deficiency, symptoms include anemia, cardiac malformations, and infections. Draw the condensed structural formula for uridine monophosphate.

Use three-letter and one-letter abbreviations to write the amino acid sequence for the peptide from each of the following mRNA sequences:

a. $A A A C C C U U G G C C$

b. $C C U C G C A G C G G C U G A$

c. $A U G C A C A A G G A A G U A C U G$

See all solutions

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How are plasmids obtained from E. coli?

Short Answer

Step by step solution

Given Information

Explanation

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Most popular questions from this chapter

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How are plasmids obtained from $E .$ coli?