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Chapter 10: Problem 5

Adhering to the convention of writing nucleotide sequences in the \(5^{\prime} \rightarrow 3^{\prime}\) direction, what is the nucleotide sequence of the DNA strand that is complementary to d-ATCGCAACTGTCACTA?

Short Answer

Expert verified
The 5\' to 3\' complementary nucleotide sequence of the DNA strand for the given sequence is TAGCGTTGACAGTAT.

Step by step solution

01

Identify the complementary base pairs

First, identify the complementary nucleotides for each nucleotide in the 5\' to 3\' direction. Use the base pairing rule, Adenine (A) pairs with Thymine (T), and Cytosine (C) pairs with Guanine (G). Therefore, for the sequence d-ATCGCAACTGTCACTA, the complementary nucleotides are T, A, G, C, G, T, T, G, A, C, A, G, T, G, A, T.
02

Formulate the complementary sequence

Next, arrange these complementary nucleotides in the same 5\' to 3\' direction to formulate the complementary DNA sequence. Therefore, the complementary nucleotide sequence for the given nucleotide sequence is TAGCGTTGACAGTAT.

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Most popular questions from this chapter

A 10 -kb DNA fragment digested with restriction endonuclease \(E c o \mathrm{RI}\) yielded fragments \(4 \mathrm{kb}\) and \(6 \mathrm{kb}\) in size. When digested with \(\mathrm{BamHI}\) fragments \(1,3.5,\) and \(5.5 \mathrm{kb}\) were generated. Concomitant digestion with both \(E c o R I\) and \(B a m H I\) yielded fragments \(0.5,1,3,\) and \(5.5 \mathrm{kb}\) in size. Give a possible restriction map for the original fragment.

Phosphate groups are also integral parts of nucleotides, with the second and third phosphates of a nucleotide linked through phosphoric anhydride bonds, an important distinction in terms of the metabolic role of nucleotides. a. What property does a phosphate group have that a nucleoside lacks? b. How are phosphoric anhydride bonds useful in metabolism? c. How are phosphate anhydride bonds an advantage to the energetics of polynucleotide synthesis?

Draw the principal ionic species of \(5^{\prime}\) -GMP occurring at pH 2.

The bases of nucleotides and polynucleotides are "information symbols." Their central role in providing information content to DNA and RNA is clear. What advantages might bases as "information symbols" bring to the roles of nucleotides in metabolism?

The DNA strand that is complementary to the template strand copied by RNA polymerase during transcription has a nucleotide sequence identical to that of the RNA being synthesized (except T residues are found in the DNA strand at sites where U residues occur in the RNA). An RNA transcribed from this nontemplate DNA strand would be complementary to the mRNA synthesized by RNA polymerase. Such an RNA is called antisense RNA because its base sequence is complementary to the "sense" mRNA. One strategy to thwart the deleterious effects of genes activated in disease states (such as cancer) is to generate antisense RNAs in affected cells. These antisense RNAs would form double- stranded hybrids with mRNAs transcribed from the activated genes and prevent their translation into protein. Suppose transcription of a cancer-activated gene yielded an mRNA whose sequence included the segment \(5^{\prime}-\) UACGGUCUAAGCUGA. What is the corresponding nucleotide sequence \(\left(5^{\prime} \rightarrow 3^{\prime}\right)\) of the template strand in a DNA duplex that might be introduced into these cells so that an antisense RNA could be transcribed from it?
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