A \(4.7 \times 10^{-2}\) mg sample of a protein is dissolved in water to make \(0.25 \mathrm{mL}\) of solution. The osmotic pressure of the solution is 0.56 torr at \(25^{\circ} \mathrm{C}\). What is the molar mass of the protein?

Short Answer

Expert verified
The molar mass of the protein is approximately 75,440 g/mol.

Step by step solution

01

Convert the given values into appropriate units

First, we need to convert the given values into appropriate units: Mass of protein = \(4.7 \times 10^{-2}\) mg = \(4.7 \times 10^{-5}\) g (1 g/1000 mg) Volume of solution = 0.25 mL = 0.00025 L (1 L/1000 mL) Osmotic pressure = 0.56 torr = 0.000736 atm (1 atm/760 torr) Temperature = 25°C = 298.15 K (273.15 + 25)
02

Calculate the number of moles of the protein using osmotic pressure formula

Using the osmotic pressure formula, we can calculate the number of moles of the protein: Osmotic pressure = (Molarity) × (R) × (Temperature) Rearrange the formula to find molarity: Molarity = Osmotic pressure / (R × Temperature) Plug in the given values: Molarity = 0.000736 atm / (0.0821 L·atm/mol·K × 298.15 K) Molarity = \(2.49 \times 10^{-6}\) mol/L
03

Find the number of moles of protein in the given volume of solution

Now, we can find the number of moles of the protein in the 0.00025 L solution using the calculated molarity: Number of moles = Molarity × Volume of solution Number of moles = \(2.49 \times 10^{-6}\) mol/L × 0.00025 L Number of moles = \(6.23 \times 10^{-10}\) mol
04

Calculate the molar mass of the protein

Finally, use the mass of the protein sample and the number of moles to find the molar mass of the protein: Molar mass = Mass of protein / Number of moles Molar mass = \(4.7 \times 10^{-5}\) g / \(6.23 \times 10^{-10}\) mol Molar mass = 75,440 g/mol The molar mass of the protein is approximately 75,440 g/mol.

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