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Chapter 32: Problem 730

Suggest a way to separate a mixture of amino acids into three fractions: monoamino monocarboxylic acids, monoamino dicarboxylic acids (the acidic amino acids), and diamino monocarboxylic acids (the basic amino acids).

Short Answer

Expert verified
To separate a mixture of amino acids into monoamino monocarboxylic acids, monoamino dicarboxylic acids (acidic amino acids), and diamino monocarboxylic acids (basic amino acids), use ion exchange chromatography. Firstly, separate monoamino monocarboxylic and diamino monocarboxylic acids using cation exchange chromatography. Then, separate monoamino dicarboxylic acids using anion exchange chromatography. Finally, you'll have three separate fractions for further analysis or experimentation.

Step by step solution

01

Understand the properties of different amino acid types

Monoamino monocarboxylic acids have one amino group(-NH2) and one carboxyl group(-COOH) which make them neutral. Monoamino dicarboxylic acids, also known as acidic amino acids, have one amino group(-NH2) and two carboxyl groups(-COOH). Diamino monocarboxylic acids, known as basic amino acids, have two amino groups(-NH2) and one carboxyl group(-COOH). These differences in the number of amino and carboxyl groups affect the overall charge and acidity or basicity of the amino acids.
02

Use Ion Exchange Chromatography

To separate the amino acids based on their differences in charge, we can use ion exchange chromatography. This technique separates compounds by their affinity for a particular charged resin. Amino acids with different charges will have different affinities for positively and negatively charged resins. We can use two different resins: a cation exchange resin that binds positively charged amino acids, and an anion exchange resin that binds negatively charged amino acids.
03

Separate monoamino monocarboxylic and diamino monocarboxylic acids using cation exchange chromatography

Firstly, set up the cation exchange chromatography column with an appropriately buffered solution. Next, load the mixture of amino acids onto the column. Diamino monocarboxylic acids (basic amino acids) will have a positive charge and will bind to the cation exchange resin, whereas monoamino monocarboxylic acids will pass through the column. Collect the eluent containing monoamino monocarboxylic acids. Elute the diamino monocarboxylic acids from the column by changing the pH or increasing the ionic strength.
04

Separate monoamino dicarboxylic acids using anion exchange chromatography

To separate the acidic amino acids, use an anion exchange chromatography column. Load the initial mixture of amino acids onto the column, and the monoamino dicarboxylic acids (acidic amino acids) will have a negative charge and bind to the anion exchange resin, while the other amino acids pass through. Collect the eluent containing the monoamino dicarboxylic acids by changing the pH or increasing the ionic strength.
05

Combine the separated fractions

Finally, you should have three separate fractions: monoamino monocarboxylic acids, monoamino dicarboxylic acids (the acidic amino acids), and diamino monocarboxylic acids (the basic amino acids). These can then be used for further analysis, purification, or experimentation.

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Most popular questions from this chapter

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