Chapter 28: Q28-36E (page 963)

Show the steps involved in a laboratory synthesis of the DNA fragmentwith the sequence CTAG.’

Short Answer

Expert verified

The original DNA will now be duplicated twice. Repeating the denatureâ€ "anneal" synthesize cycle a second time produces four DNA copies, a third time produces eight copies, and so on in an exponential series.

Step by step solution

Introduction to the Concept

Although DNA is double-stranded, only one strand is used as a transcription template at any given moment. The noncoding strand is the name given to this template strand. Because its sequence will be identical to that of the new RNA molecule, the nontemplate strand is referred to as the coding strand.

Steps involved in a laboratory synthesis of the DNA fragment

There are three steps to follows,

Tag polymerase, Mg21 particle, four deoxynucleotide triphosphate monomers (dNTPs), and an immense quantity of two short oligonucleotide ground works of roughly 20 bases each warm the twofold stranded DNA to be enhanced. Every groundwork sequence corresponds to the completion of one of the objective DNA segments. Twofold stranded DNA denatures at 95°C, splitting apart into two single strands almost instantly.
The temperature is reduced to somewhere between 37 and 50°C, allowing the ground works to temper by hydrogen-clinging to their integral grouping toward the end of each target strand, due to their generally high focus.
Tag polymerase catalyzes the extension of additional nucleotides to the two prepared DNA strands when the temperature is raised to 72A°C. When each strand's replication is complete, there are two duplicates of the original DNA strand.

Rehashing the denatureâ€"toughen orchestrate cycle a third time generates eight DNA duplicates, and so on, in an exponential structure.

The original DNA will now be duplicated twice. Repeating the denatureâ€"annealâ€"synthesize cycle a second time results in four DNA copies, a third time results in eight copies, and so on in an exponential sequence.