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Chapter 32: Problem 6

Check your knowledge of liquid chromatography detectors. Make a list of the various major types of liquid chromatography detector in order, from highest to lowest sensitivity. Which of these methods is most versatile and why?

Short Answer

Expert verified
The major types of liquid chromatography detectors in order from highest to lowest sensitivity are Electrochemical detectors, Fluorescence detectors, UV/Visible absorbance detectors, and Refractive index detectors. The most versatile of these is the UV/Visible absorbance detector since it measures any compound that absorbs in the UV or visible spectrum, making it suitable for a wide range of applications.

Step by step solution

01

Recall & Rank Detector Types

First, recall the significant types of detectors used in liquid chromatography. Note that when talking about sensitivity, it refers to a detector's ability to detect and precisely measure lower concentrations of compounds present in a mixture. Ranking in the order of highest to lowest sensitivity, the list can be as such: - Electrochemical detectors, - Fluorescence detectors, - UV/Visible absorbance detectors, and finally - Refractive index detectors.
02

Identify The Most Versatile Detector

After recalling and ranking, identify the most versatile liquid chromatography detector. Versatility relates to the ability of the detector to adapt or be adapted to many different functions or activities. For this task, UV/Visible absorbance detectors may be considered as the detector with the most versatility.
03

Justify Versatility

Now that a detector has been identified as the most versatile, provide justifications for this selection. UV/Visible absorbance detectors are versatile because they can measure any compound that absorbs light within the UV or visible spectrum. This includes a wide range of organic compounds, which makes UV/Visible absorbance detectors practical for diverse liquid chromatography applications.

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Most popular questions from this chapter

Test your knowledge of chromatographic theory - define the following terms: (a) Dead time \(\left(t_{0}\right)\) (b) Retention time \(\left(t_{\mathrm{R}}\right)\) (c) Capacity factor (k) (d) Separation factor \((\alpha)\) (e) Column efficiency \((N)\).

Calculate the resolution and efficiency of two compounds. Compounds \(X\) andY have retention times of \(18.40\) and \(20.63\) min, respectively, on a 30 \(\mathrm{cm}\) column. If the peak widths at the peak bases are \(1.11\) and \(1.21\) min, respectively, calculate: (a) The resolution factor for the two peaks. (b) The efficiency of each peak.

Test your knowledge of detector terminology. Explain what the following acronyms stand for: (a) \(\mathrm{FID}\) (b) \(\mathrm{TCD}\) (c) \(\mathrm{ECD}\) (d) DAD.

Calculate the capacity factor, resolution and number of theoretical plates of two components from a chromatogram. Quinaldine and nicotine have retention times of \(5.9\) and \(6.2\) min, respectively on a \(30 \mathrm{~cm} \times 0.25 \mathrm{~mm}\) id \(\times 0.25 \mu \mathrm{m}\) film thickness DB5 column. If the peak width of quinaldine is \(0.16\) min and for nicotine is \(0.18 \mathrm{~min}\), calculate (a) Capacity factor for quinaldine and nicotine (b) Column resolution (c) Average number of theoretical plates in the column (column efficiency, \(N\) ) per compound. Note: the retention time of the unretained component is \(1.0 \mathrm{~min}\).
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