Theof microscopic vesicles (compartments) in living cells can be estimated by infusing an indicator (HIn) into the compartment and measuring the quotientfrom the spectrum of the indicator inside the vesicle. Explain how this tells us the.

The relationship between acid pH and pKa (acid dissociation constant) in aqueous solutions is described by the Henderson-Hasselbalch equation. This equation can be used to forecast the pH of a buffer solution when the concentrations of such acid as well as its conjugate base, or the base and the related conjugate acid, are determined.

Henderson - Hasselbalch equation relates pH with p K a

The pH of the microscopic vesicles is determined by infusing an indicator into the living cells. The indicator quotientis measured spectroscopically. Using the measurement of indicator quotient obtained by spectroscopy, the Henderson – Hassel Balch equation for this case is written and pH is calculated as,

The usage of spectrochemical data in determining the pH of microscopic vesicles was explained.