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Chapter 19: Q11P (page 486)

Figure 19-6 is a Scatchard plot for the addition of 0-20nM antigen X to a fixed concentration of antibodyP=(Po=10nM)Prepare a Scatchard plot from the data in the table and find K for the reactionP+X⇌PX. The table gives measured concentrations of unbound X and the complex PX. It is recommended that the fraction of saturation should span the range ,-0.2-0.8. What is the range of the fraction of saturation for the data?

Short Answer

Expert verified

The range of the fraction of saturation for the data is from 0.29 to 0.85 and the value of K for the reactionP+X⇌PXisK=4.109M-1.

Step by step solution

01

Find the range of fraction of saturation and the value of for the given reaction:

Hence, the spreadsheet is,

Thus, the column D is the range of the fraction of saturation (from0.29 to 0.85).

Plot the graph as,

Since, the slope is K , the value ofK=4.109M-1Therefore, the value of K for the reaction isK=4.109M-1 .

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