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Chapter 26: Q19P (page 747)

(a) How can molecular exclusion chromatography be used to measure the molecular mass of a protein?

(b) Which pore size in Figure 26-15 is most suitable for chromatography of molecules with molecular mass near 100 00?

Short Answer

Expert verified

The solutions to the above questions are:

  1. Molecular exclusion chromatography
  2. 25 nm

Step by step solution

01

Molecular exclusion chromatography:

  1. Molecular exclusion chromatography – also called Gel exclusion Chromatography, a method used for separating molecules in a solution based on their size and in sometimes on their molecular weight.
  2. In this case we would measure the molecular mass of a protein by comparing the elution volume to mass standards volume.
02

Calculation of pore size:

  1. For chromatography of molecules with molecules with molecular mass near 100 000 the most suitable would be 25 nm pores.

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