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Chapter 26: Q1P (page 746)
State the effects of increasing cross-linking on an ion-exchange column.
Effects of increasing cross-linking on an ion-exchange column will be explained.
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What is electroosmosis?
Fluorescent derivatives of amino acids separated by capillary zone electrophoresis had migration times with the following order: arginine (fastest) , phenylalanine , asparagine , serine , glycine(slowest). Explain why arginine has the shortest migration time.
In the separation of proteins by hydrophobic interaction chromatography, why does eluent strength increase with decreasing salt concentration in the aqueous eluent?
Find the milliequivalents ofreleased if100mLofwere loaded on a cation exchange column in theform.
26-B. Vanadyl sulfate , FM 163.00), as supplied commercially, is contaminated withandA solution was prepared by dissolving 0.2447g of impurein 50.0 mL of water. Spectrophotometric analysis indicated that the concentration of the blueion was 0.0243M. A 5.00-mL sample was passed through a cation-exchange column loaded with. Whenfrom the 5.00-mL sample became bound to the column, thereleased required 13.03mL of 0.02274M NaOH for titration. Find the weight percent of each component) in the vanadyl sulfate.
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