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Chapter 26: Q1P (page 746)
State the effects of increasing cross-linking on an ion-exchange column.
Effects of increasing cross-linking on an ion-exchange column will be explained.
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The water-soluble vitamins niacinamide (a neutral com- pound), riboflavin (a neutral compound), niacin (an anion), and thiamine (a cation) were separated by micellar electrokinetic chromatography in 15mMborate buffer (pH8.0)with 50mMsodium dodecyl sulphate. The migration times were niacinamide (8.1min), riboflavin (13.0min), niacin (14.3min), and thiamine. What would the order have been in the absence of sodium dodecyl sulphate? Which compound is most soluble in the micelles?
(a) State the purpose of the separator and suppressor in suppressed ion chromatography. For cation chromatography, why is the suppressor an anion-exchange membrane?
The observed behavior of benzyl alcohol (C6H5CH2OH) in capillary electrophoresis is given here. Draw a graph showing the number of plates versus the electric field and explain what happens as the field increases.
Figure 26-23 shows the separation of substituted benzoates. There is a peak of unknown identity at 86.0 seconds. (a) Is the unknown a cation, neutral, or an anion? (b) Find the apparent mobility and electrophoretic mobility of the unknown peak.
Measure the width of the peak at half-height in Figure 26-32 and calculate the plate number. The capillary length to detector was 40.0 cm. Find the plate height.
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