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Chapter 26: Q20P (page 748)

A gel filtration column has a radius, y.of 0.80 cm and a length,l, of 20.0 cm

(a).Calculate the volume,Vtof the column, which is equal toπr2

(b). The interstitial volumeV0was 18.1 mL and the total volume of mobile phase was 35.8 mL.

Find.Kavfor a solute eluted at 27.4 mL.

Short Answer

Expert verified

Gel filtration column with a radius r = 0.8cm and a length l = 20cm so we will calculate the following:

(a).The volume,Vt, of the column, which is equal toπr2

(b).Kav for a solute eluted at 27.4 mL, the interstitial volume, and the total volume of the mobile phase is 35.8 mL.

Step by step solution

01

Calculate the volume of the column  by the following equation:

Vt=πr2Vt=π0.8cm2×20cmVt=40.2cm3=40.2mL

02

Calculate the by the following equation:

Kav=Vt-VoVm-Vo

Kav=27.4-18.135.8-18.1Kav=0.53

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