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Chapter 26: Q24P (page 748)
The following substances were separated on a gel filtration column. Estimate the molecular mass of the unknown.
The molecular mass is MM =
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The system in Figure 26-7 can be adapted to produce the strong acid eluent methane sulfonic acid. For this purpose, the polarity of the electrodes is reversed and the reservoir contains. The barrier membrane and the resin bed at the bottom of the figure must both be anion-exchangers loaded with.Draw this system and write the chemistry that occurs in each part.
What is the effect on injection time if you decrease the applied voltage by a factor of 2?
The observed behavior of benzyl alcohol (C6H5CH2OH) in capillary electrophoresis is given here. Draw a graph showing the number of plates versus the electric field and explain what happens as the field increases.
State three different methods to reduce electroosmotic flow. Why does the direction of electroosmotic flow change when a silica capillary is washed with a cationic surfactant?
Material balance. If you intend to measure all the anions and cations in an unknown, one sanity check on your results is that the total positive charge should equal the total negative charge. The table lists concentrations of anions and cations measured in pond water in an undergraduate experiment and expressed in mg/mL.
Find the total concentration of negative and positive charge (mol/L) to assess the quality of the analysis. What do you conclude about this analysis?
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