Learning Materials
Features
Discover
Chapter 26: Q25P (page 748)
In the separation of proteins by hydrophobic interaction chromatography, why does eluent strength increase with decreasing salt concentration in the aqueous eluent?
Decreasing salt concentration increases protein solubility in aqueous mobile phase.
Over 30 million students worldwide already upgrade their learning with Vaia!
All the tools & learning materials you need for study success - in one app.
Get started for free
Low iron concentration (as low as 0.02nM) in the open ocean limits phytoplankton growth. Preconcentration is required to determine such low concentrations. Tracefrom a large volume of seawater is concentrated onto achelating resin column. The column is then rinsed with 10mLof 1.5M high-purity water and eluted withofhigh-purity.
(a) For each sample, seawater is passed through the column for 17hours at . How much is the concentration of in the 10mLof eluate increased by this preconcentration procedure?
(b) What is the concentration of in the seawater when 57 nm is found in the nitric acid eluate?
(c) Reagent-grade concentrated nitric acid is 15.7 M and contains iron. What would be the apparent concentration of Fe (nM) in a seawater blank if reagent-grade acid were used to prepare the 1.5M eluent?
Propose a scheme for separating trimethylamine, dimethylamine, methylamine, and ammonia from one another by ion-exchange chromatography.
When the following three compounds are separated by micellar electrokinetic chromatography at, three peaks are observed. When 10mMis added to the run buffer, two of the three peaks split into two peaks, giving a total of five peaks. Explain this observation and predict which compound does not split.
To obtain the best separation of two weak acids in capillary electrophoresis, it makes sense to use the pH at which their charge difference is greatest. Prepare a spreadsheet to examine the charges of malonic and phthalic acid as a function of pH. At what pH is the difference greatest?
A polystyrene resin molecular exclusion HPLC column has a diameter of 7.8 mm and a length of 30 cm. The solid portions of the gel particles occupy 20% of the volume, the pores occupy 40%, and the volume between particles occupies 40%. (a) At what volume would totally excluded molecules be expected to emerge? (b) At what volume would the smallest molecules be expected? (c) A mixture of polyethylene glycols of various molecular masses is eluted between 23 and 27 ml. What does this imply about the retention mechanism for these solutes on the column?
What do you think about this solution?
We value your feedback to improve our textbook solutions.
Explanations 
Textbooks 
Exams 
GCSE 
IGCSE 
AS 
A Level 
International A Level 
University Admissions Tests 
Flashcards 
Vaia AI 
Notes 
Study Plans 
Study Sets 
Find a job 
Find a degree 
Student Deals 
Magazine 
Mobile App