Log out Go to App
Go to App

Learning Materials

Features

Discover

Chapter 26: Q30P (page 748)

Fluorescent derivatives of amino acids separated by capillary zone electrophoresis had migration times with the following order: arginine (fastest) , phenylalanine , asparagine , serine , glycine(slowest). Explain why arginine has the shortest migration time.

Short Answer

Expert verified

Considering that arginine is the least negative of the listed amino acids, it would move towards the anode slower and towards the cathode it would be the fastest.

Step by step solution

01

Define amino acid:

Amino acids are molecules that combine to form proteins. Amino acids and proteins are the building blocks of life.

02

Fluorescent derivatives of amino acids:

Fluorescent derivatives of amino acids are separated by capillary zone electrophoresis with the following order: arginine (fastest), phenylalanine, asparagine, serine, and glycine (slowest).

03

 Find arginine has the shortest migration time:

The listed compounds in the task are all amino acids, where only arginine contains positive side chain, whereas other amino acids have negatively charged side chains.

04

Explanation for the arginine:

Considering that arginine is the least negative of the listed amino acids, it would move towards the anode slower and towards the cathode (electroosmosis) it would be the fastest.

Unlock Step-by-Step Solutions & Ace Your Exams!

  • Full Textbook Solutions

    Get detailed explanations and key concepts

  • Unlimited Al creation

    Al flashcards, explanations, exams and more...

  • Ads-free access

    To over 500 millions flashcards

  • Money-back guarantee

    We refund you if you fail your exam.

Start your free trial

Over 30 million students worldwide already upgrade their learning with Vaia!

One App. One Place for Learning.

All the tools & learning materials you need for study success - in one app.

Get started for free

Most popular questions from this chapter

A polystyrene resin molecular exclusion HPLC column has a diameter of 7.8 mm and a length of 30 cm. The solid portions of the gel particles occupy 20% of the volume, the pores occupy 40%, and the volume between particles occupies 40%. (a) At what volume would totally excluded molecules be expected to emerge? (b) At what volume would the smallest molecules be expected? (c) A mixture of polyethylene glycols of various molecular masses is eluted between 23 and 27 ml. What does this imply about the retention mechanism for these solutes on the column?

Injection in some homebuilt capillary electrophoresis instruments is performed by raising the sample vial to create a siphon. The pressure exerted by a column of water of heightiswhereis the density of water and gis the acceleration of gravity (9.8m/s2). To what height would you need to raise the sample vial to create the necessary pressure to load the sample in 4.0 s? Is it possible to raise the inlet of this column to this height? How could you obtain the desired pressure?

Blue Dextran 2000 was eluted during gel filtration in a volume of 36.4mL from a 40 * 2cm (length diameter) column of Sephadex G-50, which fractionates molecules in the molecular mass range 1500-30000.

(a) At what retention volume would hemoglobin (molecular mass 64000 ) be expected?

(b) Suppose that radioactiveNaCI22, which is not adsorbed on the column, is eluted in a volume of 109.8 mL. What would be the retention volume of a molecule with Kav= 0.65?

(a) Explain how anions and cations can be separated by hydrophilic interaction chromatography in Figure 25-4, using the zwitterion stationary phase in Figure 25-16. Why does the gradient go from CH3CN.CH3CNto lowcontent?

(b) Explain how the charged aerosol detector used in Figure 26-4 works.

The migration time for Cl-in a capillary zone electrophoresis experiment is 17.12 min and the migration time for l-is 17.78 min. From mobilities in Table 15-1, predict the migration time of Br(The observed value is 19.6 min.)

See all solutions

Recommended explanations on Chemistry Textbooks

The Earths Atmosphere

Read Explanation

Chemical Reactions

Read Explanation

Chemistry Branches

Read Explanation

Nuclear Chemistry

Read Explanation

Making Measurements

Read Explanation

Kinetics

Read Explanation
View all explanations

What do you think about this solution?

We value your feedback to improve our textbook solutions.

Study anywhere. Anytime. Across all devices.

Sign-up for free