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Chapter 26: Q31P (page 748)

What is the principal source of zone broadening in ideal capillary electrophoresis?

Short Answer

Expert verified
  • Under ideal conditions, the lengths of injected sample and detector both contribute to zone broadening
  • Under real conditions, adsorption on the capillary and irregular flow both contribute to zone broadening

Step by step solution

01

Define capillary electrophoresis.

Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility with the use of an applied voltage.

02

Consider ideal capillary electrophoresis.

When considering ideal capillary electrophoresis we will state the principal source of zone broadening.

03

Ideal capillary conditions

In ideal capillary electrophoresis under ideal conditions, the longitudinal diffusion would be the principal source of zone broadening.

04

Result

So, under ideal conditions, the lengths of injected sample and detector both contribute to zone broadening and under real conditions, adsorption on the capillary and irregular flow both contribute to zone broadening

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Most popular questions from this chapter

Explain how neutral molecules can be separated by micellar electrokinetic chromatography. Why is this a form of chromatography? Why are micelles called a pseudostationary phase?

The system in Figure 26-7 can be adapted to produce the strong acid eluent methane sulfonic acid(CH3SO3-H+). For this purpose, the polarity of the electrodes is reversed and the reservoir containsNH4+CH3SO3-. The barrier membrane and the resin bed at the bottom of the figure must both be anion-exchangers loaded withCH3SO3-.Draw this system and write the chemistry that occurs in each part.

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(a) Draw a picture of the capillary, showing the placement of the anode, cathode, injector, and detector. Show the direction of electroosmotic flow and the direction of electrophoretic flow of a cation and an anion. Show the direction of net flow.

(b) Using Table 15-1, explain why CI-has a shorter migration time than I-Predict whether Br-will have a shorter migration time than CI-or a greater migration time than I-

(c) Why is the mobility of I-greater than that ofCI-?

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