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Chapter 26: Q31P (page 748)

What is the principal source of zone broadening in ideal capillary electrophoresis?

Short Answer

Expert verified
  • Under ideal conditions, the lengths of injected sample and detector both contribute to zone broadening
  • Under real conditions, adsorption on the capillary and irregular flow both contribute to zone broadening

Step by step solution

01

Define capillary electrophoresis.

Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility with the use of an applied voltage.

02

Consider ideal capillary electrophoresis.

When considering ideal capillary electrophoresis we will state the principal source of zone broadening.

03

Ideal capillary conditions

In ideal capillary electrophoresis under ideal conditions, the longitudinal diffusion would be the principal source of zone broadening.

04

Result

So, under ideal conditions, the lengths of injected sample and detector both contribute to zone broadening and under real conditions, adsorption on the capillary and irregular flow both contribute to zone broadening

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Most popular questions from this chapter

Measure the width of theC351- peak at half-height in Figure 26-32 and calculate the plate number. The capillary length to detector was 40.0 cm. Find the plate height.

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