Log out Go to App
Go to App

Learning Materials

Features

Discover

Chapter 26: Q34P (page 749)

Explain how neutral molecules can be separated by micellar electrokinetic chromatography. Why is this a form of chromatography? Why are micelles called a pseudostationary phase?

Short Answer

Expert verified

Short Answer is Missing in The Document

Step by step solution

01

Step1: Explain the separation of neutral molecules by micellar electrokinetic chromatography.

When there are no micelles, neutral molecules are all washed through the capillary (electroosmotic velocity). Negatively charged micelles move upstream (electrophoretic velocity), and it takes them more time to reach the detector than the neutral molecules.
Neutral molecule would spend some time being free in a solution and also dissolved in the micelles. Therefore, the net velocity would be reduced by the electroosmotic velocity.

02

Micelles as a pseudo stationary phase.

Micellar electrokinetic chromatography is a form of chromatography because in this case the micelles behave like a stationary phase because their concentration is uniform all way through the capillary.

  • Therefore, micelles are also called a pseudostationary phase

Unlock Step-by-Step Solutions & Ace Your Exams!

  • Full Textbook Solutions

    Get detailed explanations and key concepts

  • Unlimited Al creation

    Al flashcards, explanations, exams and more...

  • Ads-free access

    To over 500 millions flashcards

  • Money-back guarantee

    We refund you if you fail your exam.

Start your free trial

Over 30 million students worldwide already upgrade their learning with Vaia!

One App. One Place for Learning.

All the tools & learning materials you need for study success - in one app.

Get started for free

Most popular questions from this chapter

Would molecular exclusion, affinity, or hydrophobic interaction chromatography be most appropriate for each of the following applications?

  1. Purifying and concentrating a crude mixture of an antibody.
  2. Desalting a solution containing a 30kDa protein.
  3. Finding the molecular mass distribution of polystyrene with 15kDa average mass.
  4. Separation of cytochrome c (12 400 Da) and ribonuclease A (12 600 Da). Cytochrome c has lower surface hydrophobicity than ribonuclease A.

Injection in some homebuilt capillary electrophoresis instruments is performed by raising the sample vial to create a siphon. The pressure exerted by a column of water of heightiswhereis the density of water and gis the acceleration of gravity (9.8m/s2). To what height would you need to raise the sample vial to create the necessary pressure to load the sample in 4.0 s? Is it possible to raise the inlet of this column to this height? How could you obtain the desired pressure?

What is the effect on injection time if you decrease the applied voltage by a factor of 2?

Electroosmotic velocities of buffered solutions are shown for a bare silica capillary and one with aminopropyl groups (silica—SiOCH2CH2CH2NH2) covalently attached to the wall. A positive sign means that flow is toward the cathode. Explain the signs and relative magnitudes of the velocities.

The migration time for Cl-in a capillary zone electrophoresis experiment is 17.12 min and the migration time for l-is 17.78 min. From mobilities in Table 15-1, predict the migration time of Br(The observed value is 19.6 min.)

See all solutions

Recommended explanations on Chemistry Textbooks

Organic Chemistry

Read Explanation

Chemical Analysis

Read Explanation

Chemical Reactions

Read Explanation

Chemistry Branches

Read Explanation

Ionic and Molecular Compounds

Read Explanation

The Earths Atmosphere

Read Explanation
View all explanations

What do you think about this solution?

We value your feedback to improve our textbook solutions.

Study anywhere. Anytime. Across all devices.

Sign-up for free