Log out Go to App
Go to App

Learning Materials

Features

Discover

Chapter 26: Q41P (page 749)

The observed behavior of benzyl alcohol (C6H5CH2OH) in capillary electrophoresis is given here. Draw a graph showing the number of plates versus the electric field and explain what happens as the field increases.

Short Answer

Expert verified

When the field increases at low voltage the number of plates would increase with decreasing voltage.

Step by step solution

01

A graph showing the number of plates versus the electric field

02

Step 2:When the field increases.

  • At low voltage –the number of plates would increase with decreasing voltage.
  • Above 25000V/m the capillary could be overheating which would decrease the number of plates due to band broadening.

Unlock Step-by-Step Solutions & Ace Your Exams!

  • Full Textbook Solutions

    Get detailed explanations and key concepts

  • Unlimited Al creation

    Al flashcards, explanations, exams and more...

  • Ads-free access

    To over 500 millions flashcards

  • Money-back guarantee

    We refund you if you fail your exam.

Start your free trial

Over 30 million students worldwide already upgrade their learning with Vaia!

One App. One Place for Learning.

All the tools & learning materials you need for study success - in one app.

Get started for free

Most popular questions from this chapter

State the effects of increasing cross-linking on an ion-exchange column.

A polystyrene resin molecular exclusion HPLC column has a diameter of 7.8 mm and a length of 30 cm. The solid portions of the gel particles occupy 20% of the volume, the pores occupy 40%, and the volume between particles occupies 40%. (a) At what volume would totally excluded molecules be expected to emerge? (b) At what volume would the smallest molecules be expected? (c) A mixture of polyethylene glycols of various molecular masses is eluted between 23 and 27 ml. What does this imply about the retention mechanism for these solutes on the column?

Decomposition of dithionite(S2O42-).was studied by chromatography on an anion-exchange column eluted with 20mM tri-sodium 1, 3, 6-napthalenetrisulfonate in 90 vol%H2O/10

vol%CH3CNwith ultraviolet detection at 280 nm. A solution of sodium dithionite stored for 34 days in the absence of air gave five peaks identified asSO32-,SO42-,S2O32-,S2O42-andS2Os2-All the peaks had a negative absorbance. Explain why.

In the separation of proteins by hydrophobic interaction chromatography, why does eluent strength increase with decreasing salt concentration in the aqueous eluent?

26-D. Consider a capillary electrophoresis experiment conducted near pH9, at which the electroosmotic flow is stronger than the electrophoretic flow.

(a) Draw a picture of the capillary, showing the placement of the anode, cathode, injector, and detector. Show the direction of electroosmotic flow and the direction of electrophoretic flow of a cation and an anion. Show the direction of net flow.

(b) Using Table 15-1, explain why CI-has a shorter migration time than I-Predict whether Br-will have a shorter migration time than CI-or a greater migration time than I-

(c) Why is the mobility of I-greater than that ofCI-?
See all solutions

Recommended explanations on Chemistry Textbooks

Kinetics

Read Explanation

Chemistry Branches

Read Explanation

Ionic and Molecular Compounds

Read Explanation

Organic Chemistry

Read Explanation

Inorganic Chemistry

Read Explanation

Making Measurements

Read Explanation
View all explanations

What do you think about this solution?

We value your feedback to improve our textbook solutions.

Study anywhere. Anytime. Across all devices.

Sign-up for free