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Chapter 26: Q41P (page 749)

The observed behavior of benzyl alcohol (C6H5CH2OH) in capillary electrophoresis is given here. Draw a graph showing the number of plates versus the electric field and explain what happens as the field increases.

Short Answer

Expert verified

When the field increases at low voltage the number of plates would increase with decreasing voltage.

Step by step solution

01

A graph showing the number of plates versus the electric field

02

Step 2:When the field increases.

  • At low voltage –the number of plates would increase with decreasing voltage.
  • Above 25000V/m the capillary could be overheating which would decrease the number of plates due to band broadening.

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Most popular questions from this chapter

Figure 26-24 shows the effect on resolution of increasing voltage from 28 to120kV

(a) What is the expected ratio of migration times(t120kV/t28kV)in the two experiments? Measure the migration times for peak 1 and find the observed ratio.

(b) What is the expected ratio of plates (N120kv/N28kv)in the two experiments?

(c) What is the expected ratio of bandwidths(σ120kv/σ28kv)?

(d) What is the physical reason why increasing voltage decreases bandwidth and increases resolution?

State the effects of increasing cross-linking on an ion-exchange column.

(a) State the purpose of the separator and suppressor in suppressed ion chromatography. For cation chromatography, why is the suppressor an anion-exchange membrane?

State three different methods to reduce electroosmotic flow. Why does the direction of electroosmotic flow change when a silica capillary is washed with a cationic surfactant?

A polystyrene resin molecular exclusion HPLC column has a diameter of 7.8 mm and a length of 30 cm. The solid portions of the gel particles occupy 20% of the volume, the pores occupy 40%, and the volume between particles occupies 40%. (a) At what volume would totally excluded molecules be expected to emerge? (b) At what volume would the smallest molecules be expected? (c) A mixture of polyethylene glycols of various molecular masses is eluted between 23 and 27 ml. What does this imply about the retention mechanism for these solutes on the column?
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