Suppose that the electroosmotic mobility of a solution is +1.61×10-7m2/(V×s)) How many plates are required to separate sulfate from bromide with a resolution of 2.0? Use Table 15-1 for mobilities and Equation 26-14 for resolution.

Short Answer

Expert verified

Plates that are required to separate sulfate from bromide with a resolution of 2.0 isN=2×510plates

Step by step solution

01

Plates that are required to separate sulfate from bromide with a resolution of 2.0

From Table 15-1 we will use the following:

μepSO42-=-8.27×10-8m/Vsμapp=μeo=μepμapp=16.1×10-8+-8.27×10-8aμapp=7.83×10-8m2/VsμepBr-=-8.13×10-8m2/Vsμapp=μeo=μepμapp=16.1×10-8+-8.13×10-8μapp=7.97×10-8m2/Vs

02

Step 2:Calculate the average

Nextwewillcalculatetheaverageμandμep

μav=7.83×10-8+7.97×10-82μav=7.90×10-8m2/Vsμep=8.27×10-8-8.13×10-8μep=0.14×10-8m2/Vs

03

Step 3:Equation 26-14 for resolution

Then we will use the Equation 26-14 for resolution:

N=4×Resolutionμavμep2N=4×2.07.900.142N=2×105plates

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Most popular questions from this chapter

Fluorescent derivatives of amino acids separated by capillary zone electrophoresis had migration times with the following order: arginine (fastest) , phenylalanine , asparagine , serine , glycine(slowest). Explain why arginine has the shortest migration time.

A gel filtration column has a radius, y.of 0.80 cm and a length,l, of 20.0 cm

(a).Calculate the volume,Vtof the column, which is equal toπr2

(b). The interstitial volumeV0was 18.1 mL and the total volume of mobile phase was 35.8 mL.

Find.Kavfor a solute eluted at 27.4 mL.

(a) How many moles of analyte are present in a60.0cm×25μmsolution that occupies 1.0% of the length of a60.0cm×25μmcapillary?(b) What voltage is required to inject this many moles into a capillary in 4.0 s if the sample has 1/10 of the conductivity of background electrolyte,μmp=3.0×10-8m2/(V×s) and the sample concentration is10.0μM?

Injection in some homebuilt capillary electrophoresis instruments is performed by raising the sample vial to create a siphon. The pressure exerted by a column of water of heightiswhereis the density of water and gis the acceleration of gravity (9.8m/s2). To what height would you need to raise the sample vial to create the necessary pressure to load the sample in 4.0 s? Is it possible to raise the inlet of this column to this height? How could you obtain the desired pressure?

Figure 26-24 shows the effect on resolution of increasing voltage from 28 to120kV.

(a) What is the expected ratio of migration times (t120kV/t28kV)in the two experiments? Measure the migration times for peak 1 and find the observed ratio.

(b) What is the expected ratio of plates (N120kV/N28kV)in the two experiments?

(c) What is the expected ratio of bandwidths(σ120kV/σ28kV)?

(d) What is the physical reason why increasing voltage decreases bandwidth and increases resolution?

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