Chapter 26: Q4TY (page 738)
What is the effect on injection time if you decrease the applied voltage by a factor of 2?
Short Answer
The electro kinetic injection time is doubled, when the applied voltage is decreased by a factor of 2.
Chapter 26: Q4TY (page 738)
What is the effect on injection time if you decrease the applied voltage by a factor of 2?
The electro kinetic injection time is doubled, when the applied voltage is decreased by a factor of 2.
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Material balance. If you intend to measure all the anions and cations in an unknown, one sanity check on your results is that the total positive charge should equal the total negative charge. The table lists concentrations of anions and cations measured in pond water in an undergraduate experiment and expressed in mg/mL.
Find the total concentration of negative and positive charge (mol/L) to assess the quality of the analysis. What do you conclude about this analysis?

Consider the separation of inorganic and organic anions in Figure.
(a) What is the probable charge of pyruvate (peak 10), 2-oxovalerate(peak 16), and maleate (peak 28)? (Hint: Look at the ions around the peaks in question.)
(b) Iodide (peak 44) is a-1ion. Explain its strong retention.
d) has lower mobility than . Explain why lithium phosphate can be used at higher electric field strength than sodium phosphate to generate the same current. What is the advantage of higher field strength for this separation?
Consider a negatively charged protein adsorbed on an anion-exchange gel at pH 8.
(a) How will a gradient of eluent pH (from pH 8to some lower pH) be useful for eluting the protein? Assume that the ionic strength of the eluent is kept constant.
(b) How would a gradient of ionic strength (at constant pH) be useful for eluting the protein?
Injection in some homebuilt capillary electrophoresis instruments is performed by raising the sample vial to create a siphon. The pressure exerted by a column of water of heightiswhereis the density of water and gis the acceleration of gravity (). To what height would you need to raise the sample vial to create the necessary pressure to load the sample in 4.0 s? Is it possible to raise the inlet of this column to this height? How could you obtain the desired pressure?
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