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Chapter 26: Q7P (page 746)
What is deionized water? What kind of impurities are not removed by deionization?
Deionized water:Water that has passed through ion-exchangers to convert the present cations to protons and present anions to hydroxides
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26-D. Consider a capillary electrophoresis experiment conducted near pH9, at which the electroosmotic flow is stronger than the electrophoretic flow.
(a) Draw a picture of the capillary, showing the placement of the anode, cathode, injector, and detector. Show the direction of electroosmotic flow and the direction of electrophoretic flow of a cation and an anion. Show the direction of net flow.
(b) Using Table 15-1, explain why has a shorter migration time than Predict whether will have a shorter migration time than or a greater migration time than
(c) Why is the mobility of greater than that of?
A gel filtration column has a radius, y.of 0.80 cm and a length,l, of 20.0 cm
(a).Calculate the volume,of the column, which is equal to
(b). The interstitial volumewas 18.1 mL and the total volume of mobile phase was 35.8 mL.
Find.for a solute eluted at 27.4 mL.
Ferritin (molecular mass 450 000), transferrin (molecular mass 80 000), and ferric citrate were separated by molecular exclusion chromatography on Bio-Gel P-300. The column had a length of 37 cm and a 1.5-cm diameter. Eluate fractions of 0.65 mL were collected. The maximum of each peak came at the following fractions: ferritin, 22; transferrin, 32; and ferric citrate, 84. (That is, the ferritin peak came at an elution volume of 22 3 0.65 5 14.3 mL)Assuming that ferritin is eluted at the interstitial volume and that ferric citrate is eluted at , findfor transferrin.
Figure 26-24 shows the effect on resolution of increasing voltage from 28 to120kV.
(a) What is the expected ratio of migration times in the two experiments? Measure the migration times for peak 1 and find the observed ratio.
(b) What is the expected ratio of plates in the two experiments?
(c) What is the expected ratio of bandwidths?
(d) What is the physical reason why increasing voltage decreases bandwidth and increases resolution?
(a) The interstitial volume, , in Figure 26-15 is the volume at which the curves rise vertically at the left. Findfor the 25-nm-pore-size column. To the nearest order of magnitude, what is the smallest molecular mass of molecules excluded from this column? (b) What is the molecular mass of molecules eluted at 9.7 mL from the 12.5-nm column? (c)is the volume at which the curves drop vertically at the right. Find the largest molecular mass that can freely enter the 45-nm pores.
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