Chapter 18: Q27P (page 458)

18-27. The iron-binding site of transferrin in Figure 18-8 can accommodate certain other metal ions besides Fe and certain other anions besidesData are given in the table for the titration of transferrin ( 3.57 mg in 2.00 mL ) with 6.64 mM ${Ga}^{3 +}$ solution in the presence of the anion oxalate, $C_{2} O_{4}^{2 -}$ , and in the absence of a suitable anion. Prepare a graph similar to Figure 18-11, showing both sets of data. Indicate the theoretical equivalence point for the binding of one and two ${Ga}^{3 +}$ ions per molecule of protein and the observed end point. How many ${Ga}^{3 +}$ ions are bound to transferrin in the presence and in the absence of oxalate?

Short Answer

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The ${Ga}^{3 +}$ ions that are bound to transferrin in the absence of oxalate is zero because the slope does not change

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Find Ga3+ ions are bound to transferrin in the presence and in the absence of oxalate

The theoretical equivalence point is:

$\frac{\frac{2, n (transferrin)}{c ({Ga}^{3 +})}}{2 molGa / moltransferrin . \frac{0.00357 g transferrin}{81000 g transferrin / mol transferrin}} = 13.3 μL$

The observed end point is the intersection of lines from first 6 points and last 4 points in the following

The observed end point is $12.2 μL$ the ${Ga}^{3 +}$ ions that are bound to transferrin in the presence of oxalate is:

$\frac{12.2}{13.3} = 91.73 %$

The ${Ga}^{3 +}$ ions that are bound to transferrin in the absence of oxalate is zero because the slope does not change.

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Find Ga3+ ions are bound to transferrin in the presence and in the absence of oxalate

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