What is the difference between fluorescence and phosphorescence?

Short Answer

Expert verified

Fluorescence is the instantaneous emission of absorbed radiation.

Phosphorescence is the absorption of energy by atoms or molecules following a delayed emission of light or electromagnetic radiation.

Step by step solution

01

Fluorescence

The electrons in an atom or molecule can absorb energy in electromagnetic radiation and thus excite to the upper energy state. This upper energy state is unstable; therefore the electron likes to return to the ground state. On return, it emits an absorbed wavelength In this process of relaxation, they emit excess energy like photons. This process of relaxation is called fluorescence. Fluorescence took place much faster. Generally, it ends in about $10-5$ seconds or less of time from the moment of excitation.

02

Phosphorescence:

When molecules absorb light and go into an excited state have two possibilities. They can either release energy and immediately return to baseline or undergo other non-radiation processes. If an excited molecule undergoes a non-radiation process, it emits some energy and comes to a triplet state in which the energy is slightly less than the energy of the exited state, but is higher than the energy of the ground state. Molecules can stay a little longer in this triple state with less energy.

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Most popular questions from this chapter

Preparing standards for a calibration curve.

(a) How much ferrous ammonium sulfate(FeNH42SO426H2OFM392.15)should be dissolved in a 500mL volumetric flask withto obtain a stock solution with1000μgFe/mL?

(b) When making stock solution (a), you weighed out 3.627 g of reagent. What is the Fe concentration in?

(c) How would you prepare 250mL of standard containing containing ~1,2,3,4,5,6,7,8and10in0.1MH2SO4infrom stock solution (b) using only 5- and 10-mL Class A pipets, only 250mL volumetric flasks, and only two consecutive dilutions of the stock solution? For example, to prepare a solution with ~4μgFe/mL,, you could first dilute 15mL(=10+5mL) of stock solution up to 250 mL mLto get~(1525)(1000μgFFe/mL)=∼60μgFe/mL Then dilute 15mL of the new solution up to 250 mL again to get~(15250)(60μgFe/mL)=∼3.6μgFe/mL

What is the difference between a fluorescence excitation spectrum and a fluorescence emission spectrum? Which one resembles an absorption spectrum?

Consider a molecule that can fluoresce from the S1 state and phosphoresce from the T1 state. Which is emitted at longer wavelength, fluorescence or phosphorescence? Make a sketch showing absorption, fluorescence, and phosphorescence on a single spectrum.

A compound with molecular mass 292.16g/molwas dissolved in a 5 - mLvolumetric flask. A1.00 - mLaliquot was withdrawn, placed in a 10 - mLvolumetric flask, and diluted to the mark. The absorbance atwas in 0.427 i acuvet. The molar absorptivity at 340nm isε340=6130M-1cm-1

(a) Calculate the concentration of compound in the cuvet.

(b) What was the concentration of compound in the 5 - mL flask?

(c) How many milligrams of compound were used to make the 5 - mLsolution?

Describe a different set of dilutions to produce 3 mg Fe/mL from 1 000 mg

Fe/mL

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