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Chapter 25: Q28P (page 710)

what is the difference between extra-column volume and dwell volume? How do each of these volumes affect a chromatogram?

Short Answer

Expert verified

Extra-column volume and dwell volume

The extra-column volume influences a chromatogram by While the dwell volume delays the start of gradient elution, the peaks expand.

Step by step solution

01

definition of extra volume

Extra Volume refers to waste collected by an Acquired Subsidiary.

The extra-column volume is the volume from injection to detection excluding the column, whereas the dwell volume is the volume between the point at which the solvents are mixed and the start of the column.

02

Dwell volume

The volume required for a gradient change to reach the column or the volume difference between the point of mixing and the column head, is referred to as dwell volume.

The extra-column volume influences a chromatogram by

While the dwell volume delays the start of gradient elution, the peaks expand.

Hence,

The extra-column volume is the volume from injection to detection excluding the column, whereas the dwell volume is the volume between the point at which the solvents are mixed and the start of the column. The extra-column volume influences a chromatogram by While the dwell volume delays the start of gradient elution, the peaks expand

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Most popular questions from this chapter

Question: Literature search problem: Human serum albumin (HSA) is an important protein ingredient in cryopreservation media used in procedures such as in vitro fertilization. Search the literature for a high-performance liquid chromatography method for the determination of human serum albumin and the stabilizer N-acetyl tryptophan in medical devices.

(a) Give the citation (authors, title, journal name, year, volume, pages) for the research paper that fits the criteria of this analysis.

(b) What alternative methods could be used for analysis of human serum albumin?

(c) What type of analytical column is used for the separation?

(d) How long was the gradient? How long were the additional wash and equilibration steps within the gradient method?

(e) What parameters were assessed in the method validation?

(f) Why were particles with 300 Å pores used?

Question: Explain how to use a gradient for the first run to decide whether isocratic or gradient elution would be more appropriate.

Why are the relative eluent strengths of solvents in adsorption chromatography fairly independent of solute?

Microporous silica particles with a density of2.2g/mLand a diameter10μmof have a measured surface area of calculated the surface area of the300m2spherical silica as if it were simply solid particles what does this calculation tell you about the shape or porosity of the particles.

a. Why is high pressure needed in HPLC?

b. For a given column length , why do smaller particles give a higher plate number?

c. What is bonded phase in liquid chromatography?
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