Log out Go to App
Go to App

Learning Materials

Features

Discover

Chapter 25: Q29P (page 710)

What does it mean for a separation procedure to be ”rugged” and why is it desirable?

Short Answer

Expert verified

A separation procedure to be rugged and why is it desirable.

It is desirable because that small changed conditions do not change the final outcome of separation.

Step by step solution

01

definition of rugged

characterised by a pragmatic, unemotional attitude or point of view tenacious; unyielding.

When the separation procedure is "rugged," it is not affected by pH, temperature, gradual deterioration of the column, or minor changes in solvent composition.

It is desirable because minor changes in conditions have no effect on the final outcome of separation

02

Why is it desirable

It is desirable because that small changed conditions do not change the final outcome of separation.

Hence,

When the separation procedure is "rugged," it is not affected by pH, temperature, gradual deterioration of the column, or minor changes in solvent composition. It is desirable because that smallchanged conditions do not change the final outcome of separation.

Unlock Step-by-Step Solutions & Ace Your Exams!

  • Full Textbook Solutions

    Get detailed explanations and key concepts

  • Unlimited Al creation

    Al flashcards, explanations, exams and more...

  • Ads-free access

    To over 500 millions flashcards

  • Money-back guarantee

    We refund you if you fail your exam.

Start your free trial

Over 30 million students worldwide already upgrade their learning with Vaia!

One App. One Place for Learning.

All the tools & learning materials you need for study success - in one app.

Get started for free

Most popular questions from this chapter

(a) List ways in which the resolution between two closely spaced peaks might be changed.

(b) After optimization of an isocratic elution with several solvents, the resolution of two peaks is 1.2How might you improve the resolution without changing solvents or the kind of stationary phase?

Literature search problem: The purity of cocaine bought on the street varies dramatically. Cutting agents include levamisole, a compound normally used to kill parasites. Search the literature for a reversed-phase liquid chromatography method with diode array detection for the determination of the purity of street cocaine.

(a) Give the citation (authors, title, journal name, year, volume, pages) for the research paper that fits the criteria of this analysis.

(b) What alternative methods could be used for analysis of adulterated street cocaine?

(c) What is the range of purity of street cocaine?

(d) What type of analytical column was used for the analysis?

(e) How was the method validated?

(f) What is “robustness of an analytical method?”

A reversed-phase separation of a reaction mixture calls for isocratic elution with 48%methanol 52%water. If you want to change the procedure to use acetonitrile/water, what is a good starting percentage of acetonitrile to try?

The “rule of three” states that the retention factor for a given solute decreases approximately threefold when the organic phase increases by10%.In figure 25-12,tm=2.7min. Find k for peak 5at 40%B .predict the retention time for peak at B and compare the observed and predicted times.

25-2Why does the retention onder of peaks 2 and 3 change on the polar embedded column?

See all solutions

Recommended explanations on Chemistry Textbooks

Chemistry Branches

Read Explanation

Chemical Reactions

Read Explanation

Chemical Analysis

Read Explanation

Ionic and Molecular Compounds

Read Explanation

Making Measurements

Read Explanation

Nuclear Chemistry

Read Explanation
View all explanations

What do you think about this solution?

We value your feedback to improve our textbook solutions.

Study anywhere. Anytime. Across all devices.

Sign-up for free