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Chapter 25: Q2P (page 707)

Why are the relative eluent strengths of solvents in adsorption chromatography fairly independent of solute?

Short Answer

Expert verified

Adsorption chromatography uses a solid stationary phase and a liquid or gas mobile phase. As a result, it is independent of the solute because it is based on the difference in adsorption affinity between the adsorbent and the solvent. If the solvent's affinity is higher. A solute will be eluted from the absorbent.

Step by step solution

01

Step 1:Explaining the relative eluent chromatography of solvents.

  • Because adsorption chromatography employs a mobile phase in the liquid or solid state and a stationary phase in the solid state, the relative eluent strength of the solvent in adsorption chromatography is relatively independent of solute.
  • As a result, the solvent is competing with the solute for adsorption sites.
02

Explaining the independence of relative eluent strength.

  • Each solvent strikes a balance between adsorption on the solid surface and solvent solubility.
  • As a result, the solvent will move with the mobile phase until the equilibrium state is reached. The solvent is absorbed by the stationary phase

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Most popular questions from this chapter

Suppose that an HPLC column produces Gaussian peaks. The detector measures absorbance at 254 nm. A sample containing equal moles of compounds A and B was injected into the column. Compound A E254=2.26×104M-1cm-1has a height h=128mm and a half-width w1/2=10.1mm. Compound B E254=168×104M-1cm-1has w1/2=7.6mm. What is the height of peak B in millimeters?

  1. Use equation 25-1to estimate the length of a column required to achieve1.0×104plates if the stationary phase particles size is10.5,5.0,3.0,or1.5μm

  2. If the retention time was 20mins on the 10.0μm particle size column, what is the retention time on the 5.0,3.0,or1.5μmcolumns from part (a)? Assume that flow rate is constant for all columns.

  3. Use equation25-2to estimate the pressure of the column in (a) given that the pressure of the10.0μmcolumn was4.4Mpa

  4. If the flow rate was2.0mL/min , what is the baseline width for the peaks on 10.5,5.0,3.0,or1.5μmcolumns form part (a)?

  5. Which of these column configurations would require a UHPLC instrument?

Question: How do additives such as trienthylamine reduce tailing of certain solutes?

Question: Literature search problem: Human serum albumin (HSA) is an important protein ingredient in cryopreservation media used in procedures such as in vitro fertilization. Search the literature for a high-performance liquid chromatography method for the determination of human serum albumin and the stabilizer N-acetyl tryptophan in medical devices.

(a) Give the citation (authors, title, journal name, year, volume, pages) for the research paper that fits the criteria of this analysis.

(b) What alternative methods could be used for analysis of human serum albumin?

(c) What type of analytical column is used for the separation?

(d) How long was the gradient? How long were the additional wash and equilibration steps within the gradient method?

(e) What parameters were assessed in the method validation?

(f) Why were particles with 300 Å pores used?

A mixture of 14compounds was subjected to a reversed-phase gradient separation going from 5%to 100%acetonitrile with

a gradient time of 60min. The sample was injected at t =time. All peaks were eluted between 22and 50min.

(a) Is the mixture more suitable for isocratic or gradient elution?

(b) If the next run is a gradient, select the starting and ending %acetonitrile

and the gradient time.
See all solutions

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