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Chapter 25: Q37P (page 711)

A reversed-phase separation of a reaction mixture calls for isocratic elution with 48%methanol 52%water. If you want to change the procedure to use acetonitrile/water, what is a good starting percentage of acetonitrile to try?

Short Answer

Expert verified

Considering a vertical line (intersects each solvent line at the same elegant strength) at 48% methanol intersects the acetonitrile at 38%. Use acetonitrile/water instead of 48% methanol water 52%, the good starting percentage of acetonitrile must be 38%.

Step by step solution

01

Definition of isocratic elution

When the mobile phase has a constant concentration, isocratic elution is a term used in chromatography where the concentration of the mobile phase is constant throughout the chromatographic process.

02

Percentage of acetonitrile

Considering a vertical line (intersects each solvent line at the same elegant strength) at 48% methanol intersects the acetonitrile at 38%. Use acetonitrile/water instead of 48% methanol water 52%, the good starting percentage of acetonitrile must be 38%.

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Most popular questions from this chapter

The figure shows the separation of two enantiomers on a chiral stationary phase.


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