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Chapter 25: Q4P (page 707)

a. Why is high pressure needed in HPLC?

b. For a given column length , why do smaller particles give a higher plate number?

c. What is bonded phase in liquid chromatography?

Short Answer

Expert verified

The high pressure in HPLC is needed for usable flow rate.

Smaller particles have a higher plate number because the optimum flow rate for smaller particles is faster because the distance through which the solute must diffuse is shorter.

A bonded stationary phase is covalently attached to the surface.

Step by step solution

01

Step 1:Explanation

For the part (a), the high pressure in HPLC is needed for usable flow rate.

For the part (b), the Smaller particles have a higher plate number because the optimum flow rate is faster because the distance through which the solute must diffuse is shorter.

02

Explanation

For the part (c), a bonded stationary phase is covalently attached to the surface. For an example, silica.

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