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Chapter 23: Q25P (page 630)
Consider a chromatography column in which Find the retention factor if K = 3 and if K = 30 .
a) Retention factor when is 0.6
b) Retention factor when is is 6 .
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The weak base equilibrates between water (phase 1) and benzene (phase 2)
(a) Define the distribution coefficient, D for this system.
(b) Explain the difference between D and K the partition coefficient.
(c) Calculate D at
(d) Will D be greater or less at ? Explain why.
Why is the extraction of a metal ion into an organic solvent with more complete at higher ?
When performing liquid-liquid extraction, it is common practice to shake the separatory funnel to disperse one phase into the other.
(a) Does shaking the separatory funnel change the partition coefficient?
(b) Explain the purpose of shaking the funnel.
A layer with negligible thickness containing 10.0nmol of methanowas placed in a tube of water 5.00 cm in diameter and allowed to spread by diffusion. Using Equation 23-27, prepare a graph showing the Gaussian concentration profile of the methanol zone after 1.00,10.0, and 100 min. Prepare a second graph showing the same experiment with the enzyme rib nuclease.
23-43. An infinitely sharp zone of solute is placed at the center of a column at time . After diffusion for time , the standard deviation of the Gaussian band is . After more, at time , the standard deviation is . What will be the width after another , at time ?
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